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Epstein Barr Virus / EBV EBNA 3A Sheep Polyclonal Antibody

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SKU
AP05013PU-N
Epstein Barr Virus / EBV EBNA 3A sheep polyclonal antibody, Purified
Size:
250 ug
Conjugation:
Unconjugated
  $775.00
5 Days*
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View other "EBNA3A" antibodies

Specifications
Specifications
Product Data
Application WB
Recommended Dilution Western Blot: 0.25-1.00 µg/ml.
Positive Control: EBV infected tissues and cells.
Reactivity Epstein Barr Virus
Antibody Host Sheep
Isotype IgG
Clonality Polyclonal
Immunogen Recombinant EBNA-3A protein.
Specificity This antibody recognizes this antigen EBNA-3A (MW 145 kDa) on an immunoblot of EBV infected cells (EBV +ve).
Buffer PBS
State: Purified
State: Liquid (0.2µm sterile filtered) purified Ig fraction
Preservative: 0.08% Sodium Azide
Concentration lot specific
Purification Ammonium Sulfate Precipitation
Conjugation Unconjugated
Storage Upon receipt, store undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Predicted Protein Size 102.4 kDa
Background EBNA-3A is a latent viral nuclear protein expressed in EBV transformed lymphoblastic cell lines. It is also found in some immunoblastic lymphomas in vivo. This viral nuclear protein is essential for EBV mediated transformation of B lymphocytes. The EBNA-3A functions as a transcriptional regulator though the target genes are currently unknown. Plays an essential role for activation and immortalization of human B-cells. Represses transcription of viral promoters TP1 and Cp through interaction with host RBPJ, and inhibits EBNA2-mediated activation of these promoters. Since Cp is the promoter for all EBNA mRNAs, EBNA3A probably contributes to a negative autoregulatory control loop.
Synonyms HHV4, HHV-4
Reference Data
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Documents
Resources

file-alt Citations

Two Epstein-Barr virus (EBV) oncoproteins cooperate to repress expression of the proapoptotic tumour-suppressor Bim: clues to the pathogenesis of Burkitt's lymphoma
Anderton, Yee, Smith et al
Oncogene (2008) 27 (4), 421-33 DOI: 10.1038/sj.onc.1210668
Cited by 185
AI Summary: In this study, OriGene’s Bim (BCL2L11) shRNA lentiviral particles were used to knock down Bim expression in Burkitt’s lymphoma cells, enabling the authors to demonstrate that EBV oncoproteins EBNA3A and EBNA3C repress Bim to promote cell survival and drug resistance.
Epstein-Barr virus EBNA-3C is targeted to and regulates expression from the bidirectional LMP-1/2B promoter
Jiménez-Ramírez, Brooks, Forshell et al
J Virol (2006) 80 (22), 11200-8 DOI: 10.1128/JVI.00897-06
AI Summary: In this study, OriGene’s EBNA-3C expression plasmid (RC206307) was used to inducibly express EBNA-3C in EBV-positive cell lines, enabling the authors to investigate how EBNA-3C regulates LMP-1 and LMP-2B gene expression from their shared promoter.
Virus and cell RNAs expressed during Epstein-Barr virus replication
Yuan, Cahir-McFarland, Zhao et al
J Virol (2006) 80 (5), 2548-65 DOI: 10.1128/JVI.80.5.2548-2565.2006
AI Summary: OriGene products contributed to this study by providing the BZLF1 (Cat# SC117377) and LMP1 (Cat# SC118040) cDNA clones, which were used to analyze the expression of EBV immediate-early and latency RNAs during virus replication.
Epstein-Barr virus lytic infection contributes to lymphoproliferative disease in a SCID mouse model
Hong, Gulley, Feng et al
J Virol (2005) 79 (22), 13993-4003 DOI: 10.1128/JVI.79.22.13993-14003.2005
AI Summary: OriGene products, including the human IL-6 ELISA kit (Cat# SEK10361) and the human IL-10 ELISA kit (Cat# SEK10395), were used to quantitatively measure cytokine levels, helping demonstrate that lytic-defective EBV mutants showed reduced IL-6 and IL-10 expression compared to wild-type controls in this study.
EBNA3C coactivation with EBNA2 requires a SUMO homology domain
Rosendorff, Illanes, David et al
J Virol (2004) 78 (1), 367-77 DOI: 10.1128/jvi.78.1.367-377.2004
Cited by 58
AI Summary: In this paper, OriGene’s anti-EBNA3C antibody (TA806166) was used to detect EBNA3C protein expression, contributing to the characterization of EBNA3C’s interaction with SUMO proteins and its role in EBNA2-mediated gene activation.
Epstein-Barr virus can inhibit genotoxin-induced G1 arrest downstream of p53 by preventing the inactivation of CDK2
O'Nions, Allday
Oncogene (2003) 22 (46), 7181-91 DOI: 10.1038/sj.onc.1206838
Cited by 29
AI Summary: OriGene products, specifically the anti-p21 (TA300143) and anti-cyclin D2 (TA500127) antibodies, were used in this study to detect the levels of p21 and cyclin D2 proteins, helping to demonstrate how EBV infection prevents their normal regulation following genotoxic stress.
Showing 1-6 of 7 papers.
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