ERK1 / ERK2 Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | WB |
|---|---|
| Recommended Dilution | Western Blot: 1:500~1:1000. |
| Reactivity | Human, Mouse |
| Antibody Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | The antiserum was produced against synthesized non-phosphopeptide derived from human p44/42 MAP Kinase around the phosphorylation site of tyrosine 204 (T-E-YP-V-A). |
| Specificity | p44/42 MAP Kinase antibody detects endogenous levels of total p44/42 MAP Kinase protein. |
| Buffer | PBS(without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02% sodium azide and 50% glycerol State: Aff - Purified State: Liquid purified IgG |
| Concentration | lot specific |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
| Storage | Store the antibody at -20°C. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Background | Both p44 and p42 MAP kinases (Erk1 and Erk2) function in a protein kinase cascade that plays a critical role in the regulation of cell growth and differentiation. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine (202 and 204 of human MAP kinase Erk1 or 183 and 185 of rat Erk2) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). Both kinases are known to weakly autophosphorylate on tyrosine. |
| Synonyms | ERK-1/ERK-2, MAPK1/MAPK2, P42/P44-MAPK |
| Reference Data | |
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