BCR Rabbit Polyclonal Antibody

CAT#: AP02709PU-S

BCR rabbit polyclonal antibody, Aff - Purified

Product Images

Western Blot analysis of extracts from K562 cells using Bcr antibody

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  Immunofluorescence staining of methanol-fixed HeLa cells using BCR antibody (Red).

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunofluorescence: 1/100-1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human Bcr around the phosphorylation site of tyrosine 177 (P-F-Yp-V-N).
• Specificity: This antibody AP02709PU detects endogenous levels of total Bcr protein.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: BCR, RhoGEF and GTPase activating protein
• Database Link:
  Entrez Gene 613 Human
  UniProt ID P11274
• Background: The breakpoint cluster region protein (Bcr) is best know to be involved in genomic translocation with fusion partner Abl (Cbr-Abl) causing chronic myelogenous leukemia (CML). This 160 kDa protein contains a serine/threonine kinase domain, an SH2 binding domain, a GTP/GDP exchange domain and a C-term domain which functions as a GTPase activating protein for p21rac and CDC42. Additionally, Bcr is involved in signal transduction and can down regulate Ras mediated cell signaling.
• Synonyms: Breakpoint cluster region protein, BCR1, D22S11, NY-REN-26