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ERK1 / ERK2 pThr187/pTyr204 Rabbit Polyclonal Antibody

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SKU
AP02499PU-N
ERK1 / ERK2 pThr187/pTyr204 rabbit polyclonal antibody, Aff - Purified
Size:
100 ul 50 ul
Conjugation:
Unconjugated
  $430.00
2 Weeks*
Bulk/Customize
Specifications
Specifications
Product Data
Application IF, IHC, WB
Recommended Dilution Western Blot: 1/500~1/1000.
Immunohistochemistry: 1/50~1/100.
Immunofluorescence: 1/100~1/200.
Reactivity Human, Mouse, Rat
Antibody Host Rabbit
Clonality Polyclonal
Immunogen The antiserum was produced against synthesized phosphopeptide derived from Human p44/42 MAP Kinase around the phosphorylation site of Tyrosine 204 (T-E-Yp-V-A).
Specificity This antibody detects endogenous levels of p44/42 MAP Kinase only when phosphorylated at Tyrosine 204.
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide as preservative and 50% Glycerol as stabilizer.
State: Aff - Purified
State: Liquid purified Ig fraction.
Concentration lot specific
Purification Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Conjugation Unconjugated
Storage Store the antibody (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Stability Shelf life: One year from despatch.
Shipping Blue Ice
Background Both p44 and p42 MAP kinases (Erk1 and Erk2) function in a protein kinase cascade that plays a critical role in the regulation of cell growth and differentiation. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine (202 and 204 of human MAP kinase Erk1 or 183 and 185 of rat Erk2) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). Both kinases are known to weakly autophosphorylate on tyrosine.
Synonyms ERK-1/ERK-2, MAPK1/MAPK2, P42/P44-MAPK
Note Protocol: 1. TETE HANNKEN, et al. (2000) Am Soc Nephrol 11:1387-1397
2. Omar D. PerezNature et al. (2002) Biotechnology 20: 155 - 162
3. Jingui Yu, et al. (2005) Anesth Analg 101: 315-321
4. Hironobu Ihn et al.(2000) Immunology 165: 2149-2155
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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