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ERK1 / ERK2 pTyr204 Rabbit Polyclonal Antibody

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SKU
AP01886PU-N
ERK1 / ERK2 pTyr204 rabbit polyclonal antibody, Aff - Purified
Size:
100 ug
Conjugation:
Unconjugated
  $525.00
7 Weeks*
Bulk/Customize
Specifications
Specifications
Product Data
Application IF, IHC, WB
Recommended Dilution Western Blot: 1/500-1/1000.
Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Immunofluorescence: 1/50-1/200.
Reactivity Human, Mouse, Rat
Antibody Host Rabbit
Clonality Polyclonal
Immunogen Synthetic phosphopeptide derived from human ERK1/2 around the phosphorylation site of Tyrosine 204.
Specificity This antibody detects endogenous levels of ERK1/2 protein when phosphorylated at Tyr204.
Buffer Phosphate buffered saline (PBS), pH~7.2 containing 0.05% Sodium Azide as preservative.
State: Aff - Purified
State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
Concentration 1.0 mg/ml
Purification Affinity Chromatography using epitope-specific immunogen.
Conjugation Unconjugated
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: One year from despatch.
Shipping Blue Ice
Predicted Protein Size ~ 44,42 kDa
Database Link
Background The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at Tyrosine 204 and 187 and Threonine 177 and 160 residues mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both the Threonine 202 and Tyrosine 204 residues of ERK 1 and Threonine 185 and Tyrosine 187 residues of ERK 2 is required for full enzymatic activation. The structural consequences of dual phosphorylation in ERK 2 include active site closure, alignment of key catalytic residues that interact with ATP, and remodeling of the activation loop. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6.
Synonyms ERK-1/ERK-2, MAPK1/MAPK2, P42/P44-MAPK
Reference Data
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Documents
Resources

file-alt Citations

piR-121380 Is Involved in Cryo-Capacitation and Regulates Post-Thawed Boar Sperm Quality Through Phosphorylation of ERK2 via Targeting PTPN7
Wang, Yuan, Ali et al
Front Cell Dev Biol (2021) 9, 792994 DOI: 10.3389/fcell.2021.792994
Cited by 8
AI Summary: In this study, OriGene's PTPN7 overexpression plasmid (RC217688) and PTPN7 antibody (TA500043) were used to investigate the role of PTPN7 in piR-121380-mediated regulation of ERK2 phosphorylation and post-thawed boar sperm quality.
FigureFigureFigure
Oncogene-dependent addiction to carbohydrate-responsive element binding protein in hepatocellular carcinoma
Ribback, Che, Pilo et al
Cell Cycle (2018) 17 (12), 1496-1512 DOI: 10.1080/15384101.2018.1489182
AI Summary: OriGene’s human ChREBP-targeting siRNA (ID # SR309520) was used to suppress ChREBP expression in HCC cell lines, enabling the authors to study the effects of ChREBP silencing on cancer cell proliferation and apoptosis.
Snippet: For silencing studies, HLE and AKT/Ras cells were transfected with 50 nM siRNA targeting either human (ID # SR309520;Origene Technologies) or mouse (ID # s81679;Life Technologies, Grand Island, NY) ChREBP gene. A scrambled small interfering RNA (siRNA;ID # 4390846;Life Technologies) was used as negative control. Experiments were repeated at least three times in triplicate
Facilitatory effects of fetuin-A on atherosclerosis
Naito, Hashimoto, Watanabe et al
Atherosclerosis (2016) 246, 344-51 DOI: 10.1016/j.atherosclerosis.2016.01.037
Cited by 65
AI Summary: In this study, OriGene products such as the Human Fetuin-A cDNA clone and Fetuin-A antibody were used to analyze fetuin-A expression and its effects on vascular cells, thereby helping to elucidate fetuin-A's facilitatory role in atherosclerosis.
Role of Methionine Adenosyltransferase α2 and β Phosphorylation and Stabilization in Human Hepatic Stellate Cell Trans-Differentiation
Ramani, Donoyan, Tomasi et al
J. Cell. Physiol. (2014)
Cited by 32
AI Summary: In this study, OriGene’s MAT2A-DDK (human MAT2A-DDK-tag in pCMV6-entry) and MEF2A antibodies were used to investigate the phosphorylation and stabilization of MAT proteins during hepatic stellate cell trans-differentiation, helping to elucidate their role in liver fibrogenesis.
Snippet: MAT2A-DDK (human MAT2A-DDK-tag in pCMV6-entry) (Origene);MAT2B-HA (MAT2B-3X-HA-tag human in pReceiver-M06) (Genecopoeia, Rockville, MD). ... (Novus), MEF2A (Origene), Anti-Phosphorylated Proteins (Pan) antibody (Invitrogen), p-ERK1/2 and B- ... Cite SaveSaving
HMMR maintains the stemness and tumorigenicity of glioblastoma stem-like cells
Tilghman, Wu, Sang et al
Cancer Res. (2014)
AI Summary: OriGene's anti-HMMR antibody was used for immunohistochemistry and protein detection to assess HMMR expression in glioblastoma tissues, supporting the study's investigation of HMMR's role in maintaining stemness and tumorigenicity of glioblastoma stem-like cells.
Snippet: HMMR IHC on human GBM tissue array (GL806b, US Biomax) was performed using anti-HMMR antibody (Origene) and Vectastain Elite ABC kit (Vector Laboratories). ... Biosciences). The primary antibodies were: anti-HMMR (Origene), anti-SOX2, anti-BMI1, anti-pERK1 ... Cite SaveSaving
IHC
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