EIF3E Rabbit Polyclonal Antibody

CAT#: AP01470PU-N

EIF3E rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of eIF3e antibody (Cat.-No.: AP01470PU-N) in extracts from HepG2 cells.

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  Immunohistochemistry (IHC) analyzes of eIF3e antibody (Cat.-No.: AP01470PU-N) in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: eIF3ε antibody detects endogenous levels of eIF3ε protein. (region surrounding Val116)
• Formulation: Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 38 kDa
• Gene Name: eukaryotic translation initiation factor 3 subunit E
• Database Link:
  Entrez Gene 16341 MouseEntrez Gene 3646 Human
  UniProt ID P60228
• Background: Translation initiation in eukaryotes necessitates the assembly of an 80S ribosomal complex containing methionyl initiator tRNA (Met-tRNAiMet), which is base paired at the initiation codon (AUG, GUG) in eligible transcripts. Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that leads to 80S ribosomal assembly and initiation of translation. Eukaryotic initiation factor 3 (eIF3) is the largest family of eIFs and consists of at least 12 unique subunits in mammals. eIFε, also known as eIF p47, binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA and associates with the complex p170-eIF3.
• Synonyms: Eukaryotic translation initiation factor 3 subunit E, eIF-3 p48, INT6