PRKAR2B Rabbit Polyclonal Antibody

CAT#: AP01453PU-N

PRKAR2B rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of PKA IIβ reg antibody (Cat.-No.: AP01453PU-N) in extracts from Jurkat cells.

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  Immunohistochemistry (IHC) analyzes of PKA IIβ reg antibody (Cat.-No.: AP01453PU-N) in paraffin-embedded human cervix carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: PKA IIβ reg antibody detects endogenous levels of PKA IIβ reg protein. (region surrounding Thr109)
• Formulation: Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 46 kDa
• Gene Name: protein kinase cAMP-dependent type II regulatory subunit beta
• Database Link:
  Entrez Gene 5577 Human
  UniProt ID P31323
• Background: The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Cα, Cβ and Cγ, that each represent specific gene products. Cα and Cβ are closely related (93% amino acid sequence similarity), whereas Cγ displays 83% and 79% similarity to Cα and Cβ, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex.
• Synonyms: PRKAR2