CHRM2 Rabbit Polyclonal Antibody

CAT#: AP01304PU-N

CHRM2 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of Adenosine mAChR M2 antibody in extracts fromMCF-7 cells.

Specifications

Product Data

• Applications: ELISA, IF, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 191-240 of Human mAChR M2.
• Specificity: This antibody detects endogenous levels of mAChR M2 protein. (region surrounding Pro224)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~52 kDa
• Gene Name: cholinergic receptor muscarinic 2
• Database Link:
  Entrez Gene 1129 Human
  UniProt ID P08172
• Background: The muscarinic acetylcholine receptors (mAChR) mediate a variety of cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels. The mAChRs transducer signals by coupling to G-proteins, which then modulate several downstream effector proteins and ion channels. Five mAChR subtypes have been identified, designated M1 to M5. The five receptor subtypes show distinct patterns of tissue distribution, as well as distinct pharmacological and functional properties. The amino acid sequence of each mAChR subtype reflects a structure that is characteristic of G protein-coupled receptors, consisting of seven highly conserved transmembrane segments and a large intracellular region unique to each subtype, which constitutes the effector-coupling domain.
• Synonyms: CHRM2, mAChR M2, mAChR-M2