NANS Mouse Monoclonal Antibody [Clone ID: AT1G6]

CAT#: AM09398PU-N

NANS mouse monoclonal antibody, clone AT1G6, Purified

Product Images

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NANS antibody (1:100, 1:1000, 1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Western blot analysis The cell lysates (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NANS antibody (1:100, 1:1000, 1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Product Data

• Clone Name: AT1G6
• Applications: ELISA, WB
• Recommended Dilution: ELISA.
  Western blot (recommended dilution 1:1000).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human NANS (1-359aa) purified from E. coli
• Specificity: The antibody recognizes human NANS. Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-G affinity chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens N-acetylneuraminate synthase (NANS)
• Database Link:
  Entrez Gene 54187 Human
  UniProt ID Q9NR45
• Background: NANS (Sialic acids synthease) is an enzyme that functions in the biosynthetic pathways of sialic acids. Expressed ubiquitously, NANS enzymatically catalyzes the H2O-dependent formation of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero - D-galacto-nononic acid (KDN), both of which are sialic acids.
  NANS uses N-acetylmannosamine 6-phosphate as a substrate for Neu5Ac synthesis and mannose 6-phosphate as a substrate for KDN synthesis.
• Synonyms: SAS

Reference Data

• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Metabolic pathways