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Calpain (CAPN1+CAPN2) Mouse Monoclonal Antibody [Clone ID: 28F3]

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SKU
AM05265PU-N
Calpain (CAPN1+CAPN2) mouse monoclonal antibody, clone 28F3, Purified
Size:
100 ug
Conjugation:
Unconjugated Agarose
  $775.00
5 Days*
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Specifications
Specifications
Product Data
Clone Name 28F3
Application ELISA, IP, WB
Recommended Dilution ELISA: 0.5-1 ug/ml.
Western blot: 0.5-1 ug/ml.
Immunoprecipitation of both the native and denatured protein: 1-2 ug/ml.
Reactivity Bovine, Human
Antibody Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Calpain purified from human placenta.
Specificity This antibody specifically recognizes the 28-30 kD subunit of m-calpain and u-calpain.
React with human and bovine calpains, but does not recognize calpains from rat or mouse.
Buffer 0.02 M Sodium Phosphate, pH 7.5, 0.15 M Sodium Chloride, 50% Glycerol and 3 mM Sodium Azide as presevtative.
State: Purified
State: Liquid (sterile filtered) purified IgG fraction (> 95% pure).
Concentration lot specific
Purification Standard chromatographic techniques.
Conjugation Unconjugated
Storage Store the antibody at -20°C.
Avoid repeated freezing and thawing.
Stability Shelf life: One year from despatch.
Shipping Blue Ice
Background The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and -calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca ++ requirements that are suggested by their names. Whereas m-calpain requires millimolar (mM) levels of calcium, u-calpain is active at micromolar (uM) concentrations of Ca++. In addition to the ubiquitously expressed m- and u-calpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.
Synonyms CAPN2, CANPL2, CANP-2, CAPN1, CANPL1, CANP1
Reference Data
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Documents
Resources

file-alt Citations

Distinct Roles for μ-Calpain and m-Calpain in Synaptic NMDAR-Mediated Neuroprotection and Extrasynaptic NMDAR-Mediated Neurodegeneration
Wang, Briz, Chishti et al
J. Neurosci. (2013) 33 (48), 18880-92 DOI: 10.1523/JNEUROSCI.3293-13.2013
AI Summary: In this study, the OriGene m-calpain enzyme was used to assess its role in protein degradation during experiments investigating the distinct contributions of μ-calpain and m-calpain to synaptic and extrasynaptic NMDAR-mediated neuroprotection and neurodegeneration.
Snippet: Equal amounts of proteins (4 μg/μl) were incubated with μ-calpain (Calbiochem) or m-calpain (Origene;both at 2.4 U/ml) in the absence or presence of 2 mm calcium for 30 min at 37°C. Calpain reaction was stopped by adding Laemmli's loading buffer (final concentrations ... Cite SaveSaving
Showing 1-1 of 1 papers.
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