Calpain (CAPN1+CAPN2) Mouse Monoclonal Antibody [Clone ID: 28F3]
$775.00
5 Days*
Specifications
| Product Data | |
| Clone Name | 28F3 |
|---|---|
| Application | IP |
| Recommended Dilution | ELISA: 0.5-1 ug/ml. Western blot: 0.5-1 ug/ml. Immunoprecipitation of both the native and denatured protein: 1-2ug/ml. |
| Reactivity | Human |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Calpain purified from human placenta. |
| Specificity | This antibody specifically recognizes the 28-30 kD subunit of m-calpain and u-calpain. React with human and bovine calpains, but does not recognize calpains from rat or mouse. |
| Buffer | 20 mM Sodium Phosphate, pH 7.5, 150 mM Sodium Chloride, 50% Glycerol and 3 mM Sodium Azide as preservative. Label: Agarose State: Liquid (sterile filtered) purified IgG fraction (> 95% pure). |
| Concentration | lot specific |
| Purification | Standard chromatographic techniques. |
| Conjugation | Agarose |
| Storage | Store the antibody at -20°C. Avoid repeated freezing and thawing. |
| Stability | Shelf life: One year from despatch. |
| Shipping | Blue Ice |
| Background | The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and -calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca ++ requirements that are suggested by their names. Whereas m-calpain requires millimolar (mM) levels of calcium, u-calpain is active at micromolar (uM) concentrations of Ca++. In addition to the ubiquitously expressed m- and u-calpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux. |
| Synonyms | CAPN2, CANPL2, CANP-2, CAPN1, CANPL1, CANP1 |
| Reference Data | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.