MegaTran 2.0, plasmid DNA transfection reagent (1 mL)

Snippet: alo Alto, CA, USA). For lentiviral infection, HEK293T cells were co-transfected with recombinant lentiviral vector and pPACK Packing Plasmid Mix (System Biosciences) using Megatran reagent (TT210003, Origene, Rockville, MD, USA) to obtain lentiviruses. Viral supernatant was used to infect A549 cells in the presence of polybrene (10 μg/mL). Stable cell lines were obtained following puromycin selec

Snippet: "Sec7">Plasmid and siRNA transfectionPlasmids and siRNAs were transfected following the manufacturer’s instructions using MegaTran 2.0 plasmid DNA transfection reagent (Origene, #TT210003) or siTran 2.0 siRNA transfection reagent (Origene, # TT210001). … For plasmid transfection, DNA–lipid co;- Plasmids and siRNAs were transfected following the manufacturer’s instructions using MegaTran 2.0 plasmid DNA transfection reagent (Origene, #TT210003) or siTran

Snippet: The SW480 and AGS cells were seeded in T225 culture flask (721003, NEST, China). When the cell had reached 70% confluence, we transfected the plasmid and siRNA according to the manufacturer's instructions using MegaTran 2.0 (TT210003, Origene, USA) and siTran 2.0 (TT320002, Origene, USA)

Snippet: Mycoplasma contamination was assessed every 2 months using a mycoplasma detection kit (C0297S, Beyotime Biotechnology). The plasmids for mouse CXCR4, GPR35, and LMNB2 were purchased from Youbio (Changsha, China) and transfected according to the instructions provided with Lipofectamine 2000 (11668019, Thermo Fisher Scientific) or Megatran 2.0 (TT210003, Origene)

Snippet: (Origene, #SR323578) were transfected into HMC3 and glioma cells using siTran 2.0 siRNA transfection reagent (Origene, # reagent (Origene, #TT210003), respectively.;repeat (STR) analysis performed by Biowing Applied Biotechnology (Shanghai, China). … Cell transfection SIRT5 siRNAs (Origene, #SR323578) were transfected into HMC3 and glioma cells using siTran 2.0 siRNA transfection reagent (Origene, #TT320001) according to the manufacturer’s instructions. …