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Scrambled shRNA control in pGFP-V-RS shRNA Vector

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SKU
TR30013
29-mer Scrambled shRNA Cassette in pGFP-V-RS Vector, 5 ug
  $270.00
In Stock*
Specifications
Specifications
Product Data
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Storage Store the vector DNA at -20°C.
Stability Vector DNA is stable for at least one year from date of receipt when stored at -20°C.
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Documents
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Intellectual disability-causing mutations in KIF11 impair microtubule dynamics and dendritic arborization
Wingfield, Niese, Avchalumov et al
Nat Commun (2026)
Cited by 4
AI Summary: OriGene’s NC-GFP plasmid (TR30013) was used as a control in neuronal transfection experiments to assess the effects of KIF11 overexpression and mutations on microtubule dynamics and dendritic arborization in this study.
Snippet: vDIV16 primary hippocampal neurons culture (in 35mm Mattek dishes ) were transfected with 0.5µg of NC -GFP (Origene TR30013 ) or KIF11 -OE (Genecoepia CS -Mm03465 - Lv236 -01) for Supplementary Figure S1
AURKB and PI3K/AKT/mTOR Pathways Converge to Regulate TERT Expression
Lim, Bastian, Fung et al
iScience (2025)
Cited by 2
AI Summary: OriGene products, specifically the Human AURKB shRNA Plasmid Kit (#TL320607) and pGFP-V-RS scrambled shRNA (#TR30013), were used to silence AURKB expression in cells to study its role in regulating TERT expression.
Snippet: Cells were silenced for AURKB using the Human AURKB shRNA Plasmid Kit (#TL320607;728 Origene, Rockville, Maryland, USA) and pGFP-V-RS scrambled shRNA (#TR30013;729 Origene), following the manufacturer's guidelines. After two weeks of selection with 1 µg/mL 730 puromycin, cells were collected for western blotting and RT-qPCR analysis
Notum as a Crucial Regulator of Matrix Integrity in Dentinogenesis
Choi, Jeong, Adasooriya et al
J Cell Physiol (2025) 240 (7), e70070 DOI: 10.1002/jcp.70070
Cited by 4
AI Summary: OriGene's shRNA products (mouse Notum shRNA, TR512881, and control shRNA, TR30013) were used to generate stable cell lines for investigating the functional role of Notum in dentinogenesis in this study.
Snippet: nvitrogen), and 100 IU/mL of penicillin‐100 μg/mL of streptomycin (Invitrogen). To generate retroviral particles, shRNA against mouse Notum (TR512881) or control shRNA (TR30013) was purchased from OriGene Technologies (Rockville, MD, USA). The establishment of stable cell lines by viral transductions was performed as previously described (Choi et al. 2017). 2.6Statistical Analysis and Supporti
FigureFigureFigure
DHX9 helicase impacts on splicing decisions by modulating U2 snRNP recruitment in Ewing sarcoma cells
Frezza, Chellini, Riccioni et al
Nucleic Acids Res (2025) 53 (4) DOI: 10.1093/nar/gkaf068
Cited by 7
AI Summary: OriGene products pGFP-V-RS vector control (Cat# TR30013) and pGFP-V-RS_DHX9 shRNA vector (Cat# TG316515) were used to achieve DHX9 silencing in TC-71 cells, enabling the study of DHX9's role in alternative splicing in Ewing sarcoma.
Snippet: HX9 (Sigma Aldrich) at the final concentration of 25 nM. To obtain an additional DHX9 silencing, TC-71 cells were transfected with pGFP-V-RS vector control (shCTRL) or pGFP-V-RS_DHX9 vector (shDHX9) (Origene, Cat# TR30013 and Cat# TG316515, respectively), using Lipofectamine 2000 for 72 h. For the overexpression experiments, cells were transiently transfected with either GFP or GFP-DHX9, using Lip
FigureFigureFigure
Intellectual disability-causing mutations in KIF11 impair microtubule dynamics and dendritic arborization
Wingfield, Niese, Grover et al
Research Square (2024)
Snippet: …EB3 IMAGING AND ANALYSIS DIV14-16 Primary hippocampal mouse neurons were simultaneously transfected via combiMag and Lipofectamine with EB3-miRFP703 (Addgene #79994) and either NC-GFP (Origene TR30013) or KIF11-shRNA-C (Origene TG501174). …
Differential metabolic secretion between mdx mouse-derived spindle cell sarcomas and rhabdomyosarcomas drives tumor type development
Niba, Awano, Nishimura et al
Am J Physiol Cell Physiol (2024)
Cited by 1
AI Summary: OriGene’s shRNA constructs (sh-246 Slc1A5 and sh-control, pGFP-V-RS vector) were used in this study to knock down the alanine transporter Slc1a5 in mdx mouse tumor-derived cells, enabling the researchers to investigate the role of alanine metabolism in sarcoma development.
Snippet: any) and a control construct containing a scrambled shRNA cassette in pGFP-V-RS 245 vector TR30013 (OriGene Technologies, Inc., Rockville, MD, USA), herein referred to as sh-246 Slc1A5 and sh-control, respectively. Each construct was transfected into cultured cells 247 derived from mdx mouse tumors using the Lipo
Showing 1-6 of 80 papers.
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