pCMV-Luc Firefly Luciferase mammalian expression reporter vector

Snippet: pCMV-Luc Firefly Luciferase reporter vector (TR30004) plasmid was purchased from Origene while pGint plasmid was a kind gift from Dr. Mariano Garcia-Blanco (

Snippet: many) and was consistent with the published genotypes for these cell lines. 2.4. Cloning and Transduction The lentivirus vector coding for MACC1RFP was generated by replacing GFP in pLenti-MACC1GFP (Origene) by tagRFP (GenBank ABR08320). …

Snippet: Skip to article;The U87MG LentiRILES/133 T cells weretransduced with pre-made Renilla luciferase viral particles (R-Luc,OriGene EU, France) at MOI (multiplicity of infection) of 10 according to the procedure described above. Thereafter the dual LentiRILES/ 133 T reporter cell line was implanted in the brain of mice by stereotactic surgery using a stereotaxic frame (lab standard stereotaxic, USA) ...

Snippet: A plasmid encoding human GPR37L1 (pCMV- 88 GPR37L1) or dopamine receptor D1 (pCMV-DRD1) under the control of cytomegalovirus 89 (CMV) promoter, were obtained from Origene Technology Inc. (Cat #, GPR37L1: SC117166, 90 DRD1;RC210389, Rockville

Snippet: co-transfection for functional assay Primary HUVECs at 5 × 104 cells in passage 4 (p4) were transiently co-transfected with a 3′-UTR-SOD2 expression vector with a firefly luciferase reporter (Origene, MD, USA) and miRNA-21 mimic sequence. We used the 3′-UTR-SOD2 expression vector whose cloning region contained the entire 762 bp SOD2 3′-UTR variant 1 (NM_000636), in line with bioinform;... co-transfection for functional assay Primary HUVECs at 5 × 104 cells in passage 4 (p4) were transiently co-transfected with a 3′-UTR-SOD2 expression vector with a firefly luciferase reporter (Origene, MD, USA) and miRNA-21 mimic sequence. We used the 3′-UTR-SOD2 expression vector whose cloning region contained the entire 762 bp SOD2 3′-UTR variant 1 (NM_000636), in line with bioinform