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EGFP Escherichia coli Recombinant Protein

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SKU
TP790050
Purified fluorescent protein EGFP, with N-terminal HIS tag, expressed in E. coli, 250ug
Size:
250 ug
  $160.00
In Stock*
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Specifications
Specifications
Product Data
Species Escherichia coli
Expression Host E. coli
Expression cDNA Clone or AA Sequence
Protein Sequence
MVSKGEELFT GVVPILVELD GDVNGHKFSV SGEGEGDATY GKLTLKFICT TGKLPVPWPT LVTTLTYGVQ CFSRYPDHMK QHDFFKSAMP EGYVQERTIF FKDDGNYKTR AEVKFEGDTL VNRIELKGID FKEDGNILGH KLEYNYNSHN VYIMADKQKN GIKVNFKIRH NIEDGSVQLA DHYQQNTPIG DGPVLLPDNH YLSTQSALSK DPNEKRDHMV LLEFVTAAGI TLGMDELYK
Tag N-His
Predicted MW 26.8 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer PBS, pH 7.4, 10% glycerol
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq AAG49427
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Documents
Resources

file-alt Citations

Bright Probes, Blurred Metabolism: Navigating Fluorescent Protein Cross-Excitation in NADH FLIM
Cuala, Fuente, Cherchia et al
bioRxiv (2026)
AI Summary: OriGene’s EGFP protein (catalog #TP790050) was used as a representative fluorescent protein to assess cross-excitation and interference with NADH fluorescence lifetime imaging in this study.
Snippet: EGFP (OriGene, #TP790050) was imaged at a concentration of 150 nM, mCherry (Abcam, #ab199750) was imaged at 250 nM, and NADH (Sigma-Aldrich, #481913-1GM)
PURE makes PURE: reconstitution of the PURE cell-free system from self-synthesized proteins
Mottaghi, Maerkl
Nat Commun (2026)
Cited by 2
AI Summary: OriGene's recombinant eGFP protein (catalog no. TP790050) was used as a calibration standard to quantify protein concentrations in the PURE cell-free system experiments described in the paper.
Snippet: RFU values were converted to µg/mL based on a calibration curve. Different concentra343 tions of recombinant eGFP (OriGene, catalog no. TP790050) were prepared by dilution 344 in PBS and added to a PUREfrex reaction lacking a DNA template, as the eGFP signal is 345 amplified in PURE compared to PBS
PURE makes PURE: reconstitution of the PURE cell-free system from self-synthesized non-ribosomal proteins
Mottaghi, Maerkl
bioRxiv (2025)
Cited by 1
AI Summary: OriGene’s recombinant eGFP protein (catalog no. TP790050) was used as a standard to quantify protein synthesis in PUREfrex reactions, enabling accurate measurement of the system’s protein production capabilities.
Snippet: Different concentrations of recombinant eGFP (OriGene, catalog no. TP790050) were prepared by dilution in PBS and added to a PUREfrex reaction lacking a DNA
Photobleaching Analysis of Fluorescent Proteins in Two-Photon Microscopy at High Repetition Rates
Weng, Han
Optics Communications (2024)
Cited by 3
AI Summary: OriGene products EGFP (TP790050) and tdTomato (TP790045) were used as fluorescent protein stock solutions to investigate photobleaching behavior in two-photon microscopy experiments.
Snippet: The stock solutions of EGFP (TP790050, OriGene), tdTomato (TP790045, OriGene), Alexa Fluor 488 (A20000, ThermoFisher) and Oregon Green 488 BAPTA-1 (O6806, ThermoFisher) were diluted with a 90% glycerol (49767, Sigma) in 1× phosphate buffered saline (PBS, pH 7.4, AM9625, ThermoFisher)
Quantifying biomolecular organisation in membranes with brightness-transit statistics
Schneider, Cespedes, Karedla et al
Nat Commun (2024) 15 (1), 7082 DOI: 10.1038/s41467-024-51435-1
Cited by 6
AI Summary: OriGene’s EGFP-His protein (CAT#: TP790050) was used to prepare supported lipid bilayers for experimental validation of the brightness-transit statistics (BTS) method, enabling the study of EGFP oligomerisation and diffusion dynamics.
Snippet: The supported lipid bilayers for the experiments on EGFP-His (OriGene CAT#: TP790050;the protein carries an N-terminal His 6 tag) were prepared using spin coating of;described above to be consistent with previously acquired data and to prevent non-specific interaction possibly causing activation61,63. The supported lipid bilayers for the experiments on EGFP-His (OriGene CAT#: TP790050;the protein carries an N-terminal His6 tag) were prepared using spin coating of a lipid mix containing 4 mol% Ni-NTA-DGS in DOPC at 1 mg/mL total lipid concentration (in CH
FigureFigureFigure
Chandipura viral glycoprotein (CNV-G) promotes Gectosome generation and enables delivery of intracellular therapeutics
Zhang, Xu, Liu et al
Mol Ther (2024)
Cited by 4
AI Summary: OriGene’s purified GFP protein (Cat# TP790050) was used as a standard to quantify the amount of DmrC-GFP1-10 protein present in CNV-G gectosomes in this study.
Snippet: Purified GFP protein (Cat# TP790050, OriGENE) was used as a standard to quantify the amount 683 of DmrC-GFP1-10 in CNV-G-GFP11/DmrC-GFP1-10/NanoLuc-DmrA
Showing 1-6 of 16 papers.
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