siRNA Related Product

Snippet: A Trilencer-27 Universal scrambled negative control siRNA duplex (Origene, cat. SR30004) was used as the non-targeting control (NTC) at the same concentration. Twenty

Snippet: siRNA transfection for transient knockdown: for transient siRNA transfection, Lipofectamine RNAiMAX reagent was used at a 10pmol RNA:2μL Lipofectamine ratio following the manufacturer’s instructions with the following siRNAs: siCTRL (Origene #SR30004)

Snippet: A set of three distinct 27-mer siRNA duplexes against human NCOA4 (Cat#SR305344;referred to as siRNA-A, siRNA-B, and siRNA-C) as well as a universal 27-mer scrambled siRNA duplex (Cat#SR30004;referred to as (-)siRNA) were purchased from Origene Technologies (Rockville, MD, USA). Lipofectamine RNAiMax (Cat#13778150;ThermoFisher) was used as the transfection agent for the reverse transfection of HCEC-B4G12 and F35T cells with the anti

Snippet: A (A + B + C) directed against ADAMTS1 were used to knock down ADAMTS1 expression (A-KD) in OVCAR4, OVCAR5 and ES2 cell lines. A Universal nontargeting siRNA duplex was used as a Control (C) (OriGene Technologies, SR30004, MD, USA) in these experiments. To avoid off-target effects, the lowest ADAMTS1 siRNA concentrations were optimized for each cell line (range tested was from 1 to 10 nM)