TMEM35 (TMEM35A) (NM_021637) Human Untagged Clone
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | TMEM35 |
| Synonyms | NACHO; TMEM35; TUF-1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | NotI-NotI | |
| ACCN | NM_021637 |
| Insert Size | 504 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_021637.1, NP_067650.1 |
| RefSeq Size | 2039 bp |
| RefSeq ORF | 504 bp |
| Locus ID | 59353 |
| UniProt ID | Q53FP2 |
| Cytogenetics | Xq22.1 |
| Protein Families | Transmembrane |
| Summary | A soluble peptide released by shedding may interact with NGFR and modulate neurite outgrowth.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_021637" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC209790 | TMEM35 (Myc-DDK-tagged)-Human transmembrane protein 35 (TMEM35) | 10 ug |
$300.00
|
|
| RC209790L3 | Lenti ORF clone of Human transmembrane protein 35 (TMEM35), Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC209790L4 | Lenti ORF clone of Human transmembrane protein 35 (TMEM35), mGFP tagged | 10 ug |
$600.00
|
|
| RG209790 | TMEM35 (tGFP-tagged) - Human transmembrane protein 35 (TMEM35) | 10 ug |
$489.00
$500.00
|
|
Citations
Disentangling the mechanistic role of loop-C capping in Cys-loop receptor activation
Cymes, Grosman
Nat Commun (2025)
Cymes, Grosman
Nat Commun (2025)
Cited by 2
AI Summary: OriGene’s human NACHO (TMEM35A) cDNA in pCMV6-XL5 (#SC112910) was used in this study to investigate the mechanistic role of loop-C capping in Cys-loop receptor activation.
Snippet: …Green (University of Chicago, IL). cDNA coding human NACHO56 284 (TMEM35A;accession number: 285 Q53FP2) in pCMV6-XL5 was purchased from OriGene Technologies Inc. (#SC112910). cDNAs 286 coding the mouse 1, 1, , and subunits of the (muscle) AChR (accession numbers: P04756, 287 P09690, P02716, and P20782, respectively) in pRBG4 were provided by S. M. …
Characterizing the Ion-Conductive State of the α7-Nicotinic Acetylcholine Receptor via Single-Channel Measurements and Molecular Dynamics Simulations
Sultan, Cymes, Chen et al
bioRxiv (2025)
Sultan, Cymes, Chen et al
bioRxiv (2025)
Cited by 1
Snippet: C-3 (accession number: Q7Z5B4)16 in pcDNA3.1 was provided by W. N. Green (University of Chicago, IL);and cDNA coding human NACHO (TMEM35A;accession number: Q53FP2)17 in pCMV6-XL5 was purchased from OriGene Technologies Inc. (#SC112910). The α7-nAChR was heterologously expressed in transiently transfected adherent HEK-293 cells grown at 37°C and 5% CO2 in 35-mm cell-culture dishes.. Transfectio
Electrically silent mutants unravel the mechanism of binding-gating coupling in Cys-loop receptors
Godellas, Cymes, Grosman
Sci Adv (2024) 10 (48), eadq8048 DOI: 10.1126/sciadv.adq8048
Godellas, Cymes, Grosman
Sci Adv (2024) 10 (48), eadq8048 DOI: 10.1126/sciadv.adq8048
Cited by 5
AI Summary: OriGene’s cDNA coding human NACHO (TMEM35A; pCMV6-XL5, no. SC112910) was used in this study to investigate the mechanisms of binding-gating coupling in Cys-loop receptors.
Snippet: 3 (accession number: Q7Z5B4) (67) in pcDNA3.1 was provided by W. N. Green (University of Chicago, IL). cDNA coding human NACHO (TMEM35A;accession number: Q53FP2) (68) in pCMV6-XL5 was purchased from OriGene Technologies Inc. (no. SC112910). cDNA coding the human acid-sensing ion channel subunit 1 (ASIC1;accession number: P78348) in pCR-BluntII-TOPO was purchased from horizon (no. MHS6278-2116896
An experimental test of the nicotinic hypothesis of COVID-19
Godellas, Cymes, Grosman
Proc Natl Acad Sci U S A (2022) 119 (44), e2204242119 DOI: 10.1073/pnas.2204242119
Godellas, Cymes, Grosman
Proc Natl Acad Sci U S A (2022) 119 (44), e2204242119 DOI: 10.1073/pnas.2204242119
Cited by 11
AI Summary: In this study, OriGene's cDNA for human NACHO (TMEM35A) in pCMV6-XL5 (#SC112910) was used to facilitate the expression of nicotinic acetylcholine receptors in experiments testing the interaction between SARS-CoV-2 spike protein and these receptors.
Snippet: uman RIC-3 [accession no. Q7Z5B4 (64)] in pcDNA3.1 was provided by W. N. Green, University of Chicago, IL;cDNA coding human NACHO [TMEM35A;accession no. Q53FP2 (65)] in pCMV6-XL5 was purchased from OriGene Technologies (#SC112910);cDNA coding the human acid-sensing ion channel subunit 1 (ASIC1;accession no. P78348) in pCR-BluntII-TOPO was purchased from horizon (#MHS6278-211689646) and was sub
Probing function in ligand-gated ion channels without measuring ion transport
Godellas, Grosman
J Gen Physiol (2022) 154 (6) DOI: 10.1085/jgp.202213082
Godellas, Grosman
J Gen Physiol (2022) 154 (6) DOI: 10.1085/jgp.202213082
Cited by 6
AI Summary: In this study, OriGene’s cDNA for human NACHO (TMEM35A) in pCMV6-XL5 (#SC112910) was used to facilitate ligand-binding assays and functional analyses of pentameric ligand-gated ion channels.
Snippet: in, 2008) in pcDNA3.1 was provided by W.N. Green (University of Chicago, Chicago, IL);cDNA coding human NACHO (TMEM35A;UniProt accession no. Q53FP2;Gu et al., 2016) in pCMV6-XL5 was purchased from OriGene Technologies (#SC112910);cDNA coding the cut-and-splice (CS) chimera between the ECD of the α7-AChR from chicken and the TMD of β-GluCl from Caenorhabditis elegans in pMT3 (Cymes and Grosma
Aβ1-16 controls synaptic vesicle pools at excitatory synapses via cholinergic modulation of synapsin phosphorylation
Anni, Weiss, Guhathakurta et al
Cell Mol Life Sci (2021)
Anni, Weiss, Guhathakurta et al
Cell Mol Life Sci (2021)
Cited by 18
AI Summary: In this study, the pCMV6-XL5-TMEM35A plasmid from OriGene was used to co-transfect HEK293T cells for Ca2+ imaging and electrophysiological experiments, enabling investigation of the molecular mechanisms by which Aβ peptides modulate synaptic vesicle pools.
Snippet: …For Ca2+ imaging, HEK293T cells in a T25 flask were co-transfected with 2 μg of each pcDNA3.1-CHRNA7, (#62276, Addgene, Watertown, Massachusetts, USA, [49]), pCMV6-XL5-TMEM35A (#SC112910, OriGene Technologies, Rockville, Maryland, USA) together with 75 ng of pCVM-mCherry-N1 (#632523, Clontech, Mountain View, California, USA) plasmids. For electrophysiological studies, 1 μg of each pcDNA3.1-CHRNA7, pCMV6-XL5-TMEM35A and 75 ng pEGFP-C2 (#6083–1, Clontech, Mountain View, California,USA) were transfected. 48 h post-transfection
Showing 1-6 of 13 papers.
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