Lyve1 Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, FC, IF, IHC, WB |
|---|---|
| Recommended Dilution | ELISA: 1-15 µg/ml. Western blot: 2-5 µg/ml. FACS analysis: 3-10 µg/ml. Immunofluorescence. Immunohistochemistry on Paraffin and Frozen Sections: 0.25-4 µg/ml. |
| Reactivity | Mouse, Rat |
| Antibody Host | Rabbit |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | Highly pure (> 95%) recombinant Mouse soluble LYVE-1 (Ala24-Gly228) produced in insect cells (Cat.-No DA3524). |
| Specificity | Cross reactivity of anti-Mouse Lyve-1 with Rat tissue. The anti-Mouse Lyve-1 polyclonal antibody Cat.-No DP3513P shows a strong cross reaction with Rat Lyve-1 protein. |
| Buffer | PBS, pH 7.4, without preservatives or stabilizers State: Aff - Purified State: Lyophilized purified IgG fraction |
| Reconstitution Method | Restore in distilled sterile Water to a concentration of 0.1-1.0 mg/ml. |
| Purification | Antigen Affinity Chromatography |
| Conjugation | Unconjugated |
| Storage | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Ambient |
| Gene Name | lymphatic vessel endothelial hyaluronan receptor 1 |
| Database Link | |
| Background | LYVE-1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE-1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves. |
| Synonyms | LYVE1, CRSBP-1, CRSBP1, HAR, XLKD1 |
| Reference Data | |
| Protein Categories | Membrane Proteins |
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Citations
Autogenic-regenerated intestinal transplantation improves outcomes in short bowel syndrome
Iwaki, Ishii, Kojima et al
JCI Insight (2026)
Iwaki, Ishii, Kojima et al
JCI Insight (2026)
Niche-preserving transplantation promotes functional engraftment of intestinal organoids in rat short bowel syndrome
Endo, Sugimoto, Kuwashima et al
Cell Stem Cell (2026) 33 (1), 157-165.e6 DOI: 10.1016/j.stem.2025.12.007
Endo, Sugimoto, Kuwashima et al
Cell Stem Cell (2026) 33 (1), 157-165.e6 DOI: 10.1016/j.stem.2025.12.007
Cited by 6
AI Summary: OriGene contributed to this study by providing the rabbit anti-LYVE-1 antibody (Cat# DP3513P; RRID: AB_1004772), which was used for immunostaining to identify lymphatic endothelial cells in the transplanted intestinal tissues.
A systematic framework for the development and optimization of double immunofluorescence protocols across species: simultaneous visualization of blood and lymphatic vessels in human and murine tissue
Kerres, Zahn, Dietrich et al
SSRN Electronic Journal (2026)
Kerres, Zahn, Dietrich et al
SSRN Electronic Journal (2026)
Scanning Electron Microscopy Analysis of Lymphatic Regeneration in a Secondary Lymphedema Mouse Model: A Preliminary Study
Hayashida, Ogino, Suda et al
Lymphatics (2023) 1 (3), 237-243 DOI: 10.3390/lymphatics1030014
Hayashida, Ogino, Suda et al
Lymphatics (2023) 1 (3), 237-243 DOI: 10.3390/lymphatics1030014
Cited by 2
Snippet: Antigen-retrieved specimens were immersed in 3% H2O2 for 15 min, washed with Tris-buffered saline (TBS), and incubated with Blocking One Histo (Nacalai Tesque, Inc., Kyoto, Japan) for 10 min. After washing with TBS-0.1% Tween-20 (TBS-T), the cells were incubated for 1 h at 20 ◦C with rabbit polyclonal anti-mouse LYVE-1 antibody (2 µg/mL;DP3513P, OriGene Technologies Inc., Rockville, MD, USA). …
Scanning Electron Microscopy Analysis of Lymphatic Regeneration in a Secondary Lymphedema Mouse Model
Hayashida, Ogino, Suda et al
Preprint.org (2023)
Hayashida, Ogino, Suda et al
Preprint.org (2023)
Cited by 1
Snippet: Under inflammatory conditions including lymphatic disorders, bone marrow-derived myeloid cells often express lymphatic endothelial cell (LEC) markers called LEC Save;After washing with TBS-0.1% Tween-20 (TBS-T), the cells were incubated for 1 h at 20°C with rabbit polyclonal antimouse LYVE-1 antibody (2 μg/mL;DP3513P, OriGene Technologies Inc., Rockville, MD, USA). …
Neuroimmune cardiovascular interfaces control atherosclerosis
Mohanta, Peng, Li et al
Nature (2022) 605 (7908), 152-159 DOI: 10.1038/s41586-022-04673-6
Mohanta, Peng, Li et al
Nature (2022) 605 (7908), 152-159 DOI: 10.1038/s41586-022-04673-6
Cited by 242
AI Summary: In this study, the OriGene Lyve1 antibody (DP3513P) was used to identify lymphatic endothelial cells in mouse arterial tissue, helping to characterize neuroimmune cardiovascular interfaces involved in atherosclerosis.
Snippet: … As plaques lack innervation, the effects of neuronal control Save;A85-1, BD, 1:50), anti-mouse MECA32 (550563, BD, 1:500), Lyve1 (DP3513P, OriGene, 1:100), anti-mouse PNAd (MECA-79, BD, 1:100), anti-mouse collagen IV (2150-1470, Serotec, 1:500), anti-mouse ERTR7 (T-2109, BMA, 1:100), anti-mouse CXCL13 (AF470
Showing 1-6 of 20 papers.
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