Candida albicans Rabbit Polyclonal Antibody

Snippet: Without prior antigen-retrieval, they were stained with 80 µL primary antibody mix consisting of PBS, 1% BSA, 0.3% Triton X-100, 10 µg/mL anti-_Candida_ (polyclonal rabbit, BP1006, Acris Antibodies/OriGene Technologies) and 5 µg/mL AlexaFluor 647-coupled anti Ly6G (clone 1A8, #127610, Biolegend) overnight at 4°C inside a humid chamber. Samples were washed for three times and incubated with sec

Snippet: A;Serva) for 60 min. C. albicans was stained using rabbit anti-C. albicans antibody (1:600 in PBS with 0.1% BSA and 0.05% saponin, BP1006, Acris Antibodies) overnight, together with ISG15 using mouse anti-ISG15 IgG (1:100 in PBS with 0.1% BSA and 0.05% saponin, sc-166755, Santa Cruz Biotechnology). Afterwards, samples were washed with PB

Snippet: Mouse Fc-block (eBioscience/Thermo Fischer GmbH, Darmstadt, Germany) was added for 30 min, and rabbit-anti-Candida antibody (BP1006, Acris antibodies) was

Snippet: oat serum in PBS with 0.3% Triton-X100 for 90 min and, without 569 prior antigen-retrieval, overnight stained with 80 µl primary antibody mix including PBS, 1% 570 BSA, 0.3% Triton-X100, 10 µg/ml anti-Candida (polyclonal rabbit, BP1006, Acris 571 Antibodies/OriGene Technologies) and 5 µg/ml AlexaFluor-647-coupled anti-Ly6G (clone 572 1A8, #127610, Biolegend) at 4 °

Snippet: lized (PBS with 1% BSA and 0.05% saponin) for 30 min at RT. C. albicans was stained using rabbit anti-C. albicans antibody (1:600 in PBS with 0.1% BSA and 0.1% saponin, rabbit anti-Candida, BP1006, Acris Antibodies) overnight, washed with PBS, and incubated with a secondary goat anti-rabbit AlexaFluor488 (1:500 in PBS with 0.1% BSA and 0.1% saponin, Invitrogen A32731) for 1 h in the dark at RT