Antibody Specificity Validation Technology
Under current specifications, ~17,000 samples were spotted in duplicates on a single nitrocellulose slide. These include 17,000 different overexpression lysates and 708 positive and negative control samples. Serial diluted mouse, human and rabbit IgG mixture as positive controls were spotted on three subarrays highlighted in redboxes. Subarrays highlighted in yellow boxes contain serial diluted HEK293T cell lysates as negative controls. All the subarrays contain standard positive and negative controls as shown in the figure.
UltraMAB® specificity validation: 17K high density protein microarray