pEX-N-His Bacterial Expression Vector

Snippet: 3N) proteins were recombinantly produced. Briefly, the DNA sequences of wildtype and quadruple mutant of Anx5 were codon optimized, synthesized, and cloned into pEX-N-His expression vector (PS100030, Origene, Rockville, MD, USA). The plasmids were transformed into BL21(DE3)-competent cells to produce recombinant proteins, which were purified using His-tag affinity chromatography (Nickel column, Hi

Snippet: UBE2D3 protein levels were analysed by western blot. Protein expression in bacteria and extraction. The cDNAs of Ube2d3 mutants (wild-type, S138A, S138E, S138D) were cloned into the pET-N-His (Origene) vector containing a 6xHis N-terminal tag. E. coli BL21

Snippet: The selected colonies were checked by DNA sequence verification. The cDNA encoding full- length XRCC1 and residues 166-436 was amplified by PCR and inserted into the bacterial expression plasmid pEX-N-His (OriGene, Rockville, MD) using AscI-RsrII restriction

Snippet: Human VASP was cloned into pEXN-His (OriGene)

Snippet: Briefly, we purified cDNA fragment of full-length human NRGN gene by restriction enzyme digestion (Sgf I and Mlu I) and agarose gel purification from human cDNA ORF clone (RC201209, Origene, Rockville, MD), and then ligated into bacterial expression vector pEX ... Cite SaveSaving