pEX-N-His-GST Bacterial Expression Vector

Snippet: 20,21]. Our group has been studying xanthones for their in vitro and in vivo anticancer activity, and our data suggests that xanthones promote apoptosis in PCa cells, degrade AR, and slow prostate tumorigenesis in mice [22,23,24,25,26,27,28,29]. This study was undertaken to test our hypothesis that α-mangostin, the most abundant mangosteen xanthone, promotes the ubiquitination and degradation of

Snippet: Expression of recombinant Tau 123 and 224 Tau 123 (aa 1-123 of Tau F) and Tau 224 (aa 1-224) were PCR amplified using primers containing 5′ Sgf I and 3′ Not I sites with cDNA for Tau F (RC213312, Origene) as tem- plate

Snippet: DTT. The coding sequence of full-length rat epsin was obtained from Addgene (pCDNA3.1-Epsin1;plasmid 22225) and was sub-cloned into the pEX-N-His 6 -GST vector (Origene) for expression in E. coli BL21[DE3]. Purification ... Cite SaveSaving

Snippet: and 1 µg/ml LF). 6His-GST-fused rNlrp1 proteins were expressed in plasmid pEX-N-His-GST (cat# PS100028, Origene, Rockville, MD) and purified on glutathione-Sepharose columns by standard methods. High affinity anti-HA