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Brca1 Mouse siRNA Oligo Duplex (Locus ID 12189)

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SKU
SR423219
Brca1 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each
  $518.00
In Stock*

Frequently Bought Together (3)

TT320001 siTran 2.0 siRNA transfection reagent (0.5ml)
500 ul
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SR30002 Trilencer-27 Fluorescent-labeled transfection control siRNA duplex - 1 nmol
1 nmol
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TT300007S A non-viral, non-electroporation, non-liposomal transfection kit using engineered mammalian proteins, tailored for high-efficiency delivery into a wide range of primary cells and difficult to transfect cell lines such as immune cells, stem cells, and neuronal cells.
20 ul
$265.00

Other products for "Brca1 or NM_009764"

Specifications
Specifications
Product Data
Purity HPLC purified
Quality Control Tested by ESI-MS
Sequences Available with shipment
Components
Brca1 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 12189)
Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmol
Included - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml
Stability One year from date of shipment when stored at -20°C.
Shipping Ambient
# of Transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).
Note Single siRNA duplex (10nmol) can be ordered.
Reference Data
RefSeq NM_009764
UniProt ID P48754
Summary E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The E3 ubiquitin-protein ligase activity is required for its tumor suppressor function. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Regulates centrosomal microtubule nucleation. Required for normal cell cycle progression from G2 to mitosis. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Contributes to homologous recombination repair (HRR) via its direct interaction with PALB2, fine-tunes recombinational repair partly through its modulatory role in the PALB2-dependent loading of BRCA2-RAD51 repair machinery at DNA breaks. Component of the BRCA1-RBBP8 complex which regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage via BRCA1-mediated ubiquitination of RBBP8. Acts as a transcriptional activator (By similarity). Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation.UniProtKB/Swiss-Prot Function
Performance Guaranteed OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency.

For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required).
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Documents
Resources

file-alt Citations

Activation of TGR5 protects blood brain barrier via the BRCA1/Sirt1 pathway after middle cerebral artery occlusion in rats
Liang, Matei, McBride et al
J. Biomed. Sci. (2020) 27 (1), 61 DOI: 10.1186/s12929-020-00656-9
Cited by 44
AI Summary: OriGene products, specifically TGR5-siRNA and BRCA1-siRNA, were used in this study to selectively silence TGR5 and BRCA1 genes in rats, enabling the researchers to investigate the roles of these genes in protecting the blood-brain barrier after stroke.
Snippet: Intracerebroventricular siRNA injection Three different formats of TGR5-siRNA or BRCA1- siRNA (OriGene Technologies) were diluted with trans- fection reagent (entranser ,Engreen Biosystem) and were injected 48h before MCAO by intracerebroventricular;Three different formats of TGR5-siRNA or BRCA1-siRNA (OriGene Technologies) were diluted with transfection reagent (entranser ,Engreen Biosystem) and were injected 48 h before MCAO by intracerebroventricular injection (ICV) as previously described [21, 22]. … The TGR5-siRNA, BRCA1-siRNA mixture or scramble-siRNA (100 pmol in 5 μL) was delivered into the ipsilateral ventricle with a Hamilton syringe (Microliter 701, Hamilton Company, Reno, NV) and administered over 5 min. …
FigureFigureFigure
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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