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Top2a Mouse siRNA Oligo Duplex (Locus ID 21973)

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SKU
SR422969
Top2a (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each
  $518.00
In Stock*

Frequently Bought Together (3)

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SR30002 Trilencer-27 Fluorescent-labeled transfection control siRNA duplex - 1 nmol
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TT300007S A non-viral, non-electroporation, non-liposomal transfection kit using engineered mammalian proteins, tailored for high-efficiency delivery into a wide range of primary cells and difficult to transfect cell lines such as immune cells, stem cells, and neuronal cells.
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Other products for "Top2a or NM_011623"

Specifications
Specifications
Product Data
Purity HPLC purified
Quality Control Tested by ESI-MS
Sequences Available with shipment
Components
Top2a (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 21973)
Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmol
Included - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml
Stability One year from date of shipment when stored at -20°C.
Shipping Ambient
# of Transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).
Note Single siRNA duplex (10nmol) can be ordered.
Reference Data
RefSeq NM_011623
UniProt ID Q01320
Synonyms Top-2
Summary Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (PubMed:24321095).UniProtKB/Swiss-Prot Function
Performance Guaranteed OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency.

For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required).
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Documents
Resources

file-alt Citations

Identification of Bisindolylmaleimide IX as a potential agent to treat drug-resistant BCR-ABL positive leukemia
Zhang, Jia, Ao et al
Oncotarget (2016) 7 (43), 69945-69960 DOI: 10.18632/oncotarget.11566
Snippet: To knock down TopIIa or B-Raf, BaF3 cells (3 x 106 cells per sample) were pelleted and resuspended in 100 μl room temperature Nucleofector Solution (LONZA, Swiss), which were then mixed with 100 nM siRNA (Origene, USA)
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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