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Adgrf4 Mouse siRNA Oligo Duplex (Locus ID 78249)

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SKU
SR419262
Adgrf4 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each
  $518.00
In Stock*

Frequently Bought Together (3)

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Other products for "Adgrf4 or NM_001289499"

Specifications
Specifications
Product Data
Purity HPLC purified
Quality Control Tested by ESI-MS
Sequences Available with shipment
Components
Adgrf4 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 78249)
Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmol
Included - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml
Stability One year from date of shipment when stored at -20°C.
Shipping Ambient
# of Transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).
Note Single siRNA duplex (10nmol) can be ordered.
Reference Data
RefSeq NM_001289499, NM_001289500, NM_001289501, NM_030067
UniProt ID Q9D2L6
Synonyms 4632435A09Rik; AV239010; Gpr115
Summary Orphan receptor.UniProtKB/Swiss-Prot Function
Performance Guaranteed OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency.

For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required).
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Documents
Resources

file-alt Citations

G-protein coupled receptor Gpr115 (Adgrf4) is required for enamel mineralization mediated by ameloblasts
Chiba, Yoshizaki, Saito et al
J. Biol. Chem. (2020) 295 (45), 15328-15341 DOI: 10.1074/jbc.RA120.014281
AI Summary: OriGene products, specifically the Trilencer-27 Universal scrambled negative control siRNA duplex (siNeg) and Adgrf4 Mouse siRNA Oligo Duplex (siGpr115), were used to knock down Gpr115 expression in CLDE cells to study its role in enamel mineralization.
Snippet: For RT-qPCR and mineralization assay examinations, CLDE cells were cultured until 80% confluency and transfected with Trilencer-27 Universal scrambled negative control siRNA duplex (Origene, siNeg), Adgrf4 Mouse siRNA Oligo Duplex (Origene, siGpr115), and
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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