Nuak2 Mouse siRNA Oligo Duplex (Locus ID 74137)
$518.00
In Stock*
Specifications
| Product Data | |
| Purity | HPLC purified |
|---|---|
| Quality Control | Tested by ESI-MS |
| Sequences | Available with shipment |
| Components |
Nuak2 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 74137)Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmolIncluded - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml
|
| Stability | One year from date of shipment when stored at -20°C. |
| Shipping | Ambient |
| # of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
| Note | Single siRNA duplex (10nmol) can be ordered. |
| Reference Data | |
| RefSeq | NM_001195025, NM_028778 |
| UniProt ID | Q8BZN4 |
| Synonyms | 1200013B22Rik; mKIAA0537; Omphk2; Snark |
| Summary | Stress-activated kinase involved in tolerance to glucose starvation. Induces cell-cell detachment by increasing F-actin conversion to G-actin. Expression is induced by CD95 or TNF-alpha, via NF-kappa-B. Protects cells from CD95-mediated apoptosis and is required for the increased motility and invasiveness of CD95-activated tumor cells. Able to phosphorylate 'Ser-464' of LATS1 (By similarity).UniProtKB/Swiss-Prot Function |
| Performance Guaranteed | OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency. For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required). |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Citations
The MicroRNA miR-696 is regulated by SNARK and reduces mitochondrial activity in mouse skeletal muscle through Pgc1α inhibition
Queiroz, Lessard, Ouchida et al
Mol Metab (2021) 51, 101226 DOI: 10.1016/j.molmet.2021.101226
Queiroz, Lessard, Ouchida et al
Mol Metab (2021) 51, 101226 DOI: 10.1016/j.molmet.2021.101226
Cited by 29
AI Summary: In this study, OriGene’s SNARK overexpression plasmid (Cat# MR207779) and SNARK shRNA (Cat# TL513527) were used to manipulate SNARK levels in C2C12 cells, enabling the authors to demonstrate SNARK’s regulatory effect on miR-696 expression and its downstream impact on mitochondrial activity in skeletal muscle.
E3 ubiquitin ligase RNF126 affects bladder cancer progression through regulation of PTEN stability
Xu, Ju, Xiong et al
Cell Death Dis (2021) 12 (3), 239 DOI: 10.1038/s41419-021-03521-1
Xu, Ju, Xiong et al
Cell Death Dis (2021) 12 (3), 239 DOI: 10.1038/s41419-021-03521-1
Cited by 55
AI Summary: In this study, OriGene products including RNF126 cDNA clone (Cat# RC210372) and PTEN shRNA (Cat# TL309919) were used to manipulate gene expression and investigate the roles of RNF126 and PTEN in bladder cancer progression.
Snippet: E3 ubiquitin ligase RNF126 (ring finger protein 126) is highly expressed in various cancers and strongly associated with tumorigenesis. However, its specific function in bladder cancer (BCa) is still debatable. Here, we found that RNF126 was significantly upregulated
The MicroRNA miR-696 is Regulated by SNARK and Reduces Mitochondrial Activity in Mouse Skeletal Muscle Through Pgc1α Inhibition
Queiroz, Lessard, Ouchida et al
Molecular Metabolism (2021) 51, 101226 DOI: 10.1016/j.molmet.2021.101226
Queiroz, Lessard, Ouchida et al
Molecular Metabolism (2021) 51, 101226 DOI: 10.1016/j.molmet.2021.101226
Cited by 29
AI Summary: In this study, OriGene's siRNA products targeting SNARK (SR30004, SR418439B) were used to knock down SNARK expression in C2C12 cells, helping to elucidate SNARK's role in regulating miR-696 and mitochondrial activity in mouse skeletal muscle.
Snippet: ransfection, C2C12 cells were transfected on day 3 of differentiation using Lipofectamine RNAiMAX reagent (Invitrogen-Life Technologies). siRNA against SNARK or scrambled sequences were obtained from Origene (SR30004, SR418439B). … siRNA against SNARK or scrambled sequences were obtained from Origene (SR30004, SR418439B). The miR-696 mimic, miR-696 ASO, and scrambled sequences were synthesized by miRIDIAN (Dharmacon-GE). 2.9 Reporter Assay;siRNA against SNARK or scrambled sequences were obtained from Origene (SR30004, SR418439B)
The AMPK-related kinase SNARK regulates muscle mass and myocyte survival
Lessard, Rivas, So et al
J. Clin. Invest. (2015)
Lessard, Rivas, So et al
J. Clin. Invest. (2015)
Examination of potential mechanisms linking AMPK to inhibition of IL-6 signalling
Speirs
Thesis (2017)
Speirs
Thesis (2017)
Showing 1-6 of 6 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
