OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Pdk4 Mouse siRNA Oligo Duplex (Locus ID 27273)

Be the first to review this product
SKU
SR413673
Pdk4 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each
  $518.00
In Stock*

Frequently Bought Together (3)

TT320001 siTran 2.0 siRNA transfection reagent (0.5ml)
500 ul
$280.00
SR30002 Trilencer-27 Fluorescent-labeled transfection control siRNA duplex - 1 nmol
1 nmol
$142.00
TT300007S A non-viral, non-electroporation, non-liposomal transfection kit using engineered mammalian proteins, tailored for high-efficiency delivery into a wide range of primary cells and difficult to transfect cell lines such as immune cells, stem cells, and neuronal cells.
20 ul
$265.00

Other products for "Pdk4 or NM_013743"

Specifications
Specifications
Product Data
Purity HPLC purified
Quality Control Tested by ESI-MS
Sequences Available with shipment
Components
Pdk4 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 27273)
Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmol
Included - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml
Stability One year from date of shipment when stored at -20°C.
Shipping Ambient
# of Transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).
Note Single siRNA duplex (10nmol) can be ordered.
Reference Data
RefSeq NM_013743
UniProt ID O70571
Synonyms AV005916
Summary Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism in response to prolonged fasting and starvation. Plays an important role in maintaining normal blood glucose levels under starvation, and is involved in the insulin signaling cascade. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. In the fed state, mediates cellular responses to glucose levels and to a high-fat diet. Regulates both fatty acid oxidation and de novo fatty acid biosynthesis. Plays a role in the generation of reactive oxygen species. Protects detached epithelial cells against anoikis. Plays a role in cell proliferation via its role in regulating carbohydrate and fatty acid metabolism.UniProtKB/Swiss-Prot Function
Performance Guaranteed OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency.

For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required).
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources

file-alt Citations

Upregulation of the AMPK-FOXO1-PDK4 pathway is a primary mechanism of pyruvate dehydrogenase activity reduction in tafazzin-deficient cells
Liang, Ralph-Epps, Schmidtke et al
Sci Rep (2024) 14 (1), 11497 DOI: 10.1038/s41598-024-62262-1
Cited by 12
AI Summary: OriGene’s siRNAs targeting PDK4 (SR413673) and scrambled negative control siRNA (SR30004) were used to selectively knock down PDK4 expression, enabling the authors to investigate its role in pyruvate dehydrogenase inhibition in tafazzin-deficient cells.
Snippet: ernatants containing protein were used for immunoblotting analysis. siRNA construct and incubation siRNAs targeting PDK4 (SR413673) and scrambled negative control siRNA (SR30004) were purchased from Origene. siRNA was transfected using TransIT-X2 Dynamic Delivery System (Mirus) on the 2nd day after cell seeding and cultured for 24 h before collection. RNA extraction and real-time qPCR analysi
FigureFigureFigure
Upregulation of the AMPK-FOXO1-PDK4 pathway is a primary mechanism of pyruvate dehydrogenase activity reduction and leads to increased glucose uptake in tafazzin-deficient cells
Liang, Ralph-Epps, Schmidtke et al
bioRxiv (2024)
Cited by 2
Snippet: siRNAs targeting PDK4 (SR413673) and scrambled negative control siRNA (SR30004) were purchased from Origene. siRNA was transfected using TransIT-X2 Dynamic
Showing 1-2 of 2 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products