Sdhc Mouse siRNA Oligo Duplex (Locus ID 66052)
$518.00
3 Weeks*
Specifications
| Product Data | |
| Purity | HPLC purified |
|---|---|
| Quality Control | Tested by ESI-MS |
| Sequences | Available with shipment |
| Components |
Sdhc (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 66052)Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmolIncluded - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml
|
| Stability | One year from date of shipment when stored at -20°C. |
| Shipping | Ambient |
| # of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
| Note | Single siRNA duplex (10nmol) can be ordered. |
| Reference Data | |
| RefSeq | NM_025321 |
| UniProt ID | Q9CZB0 |
| Synonyms | 0610010E03Rik; AI316496; AU019277 |
| Summary | Membrane-anchoring subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).UniProtKB/Swiss-Prot Function |
| Performance Guaranteed | OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency. For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required). |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Citations
Horizontal transfer of whole mitochondria restores tumorigenic potential in mitochondrial DNA-deficient cancer cells
Dong, Kovarova, Bajzikova et al
Elife (2017) 6 DOI: 10.7554/eLife.22187
Dong, Kovarova, Bajzikova et al
Elife (2017) 6 DOI: 10.7554/eLife.22187
AI Summary: OriGene’s shRNA products targeting NDUFV1 and SDHC were used to knock down these mitochondrial complex subunits in B16ρ(0) cells, demonstrating their essential role in tumor formation by assessing the effect of gene silencing on tumorigenic potential.
Snippet: H2B plasmid (Evrogen) followed by clonal selection. RNAi was used to knock down 347 NDUFV1 or SDHC subunits, using two different shRNAs (OriGene) for each protein. In 348 brief, cells were transfected with shRNA using a standard protocol and inspected by WB ... All 3 versions Cite SaveSaving
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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