Id1 Mouse siRNA Oligo Duplex (Locus ID 15901)

SKU
SR403015
Id1 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each
  $518.00
In Stock*
Specifications
Specifications
Product Data
Purity HPLC purified
Quality Control Tested by ESI-MS
Sequences Available with shipment
Components
Id1 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 15901)
Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmol
Included - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml
Stability One year from date of shipment when stored at -20°C.
Shipping Ambient
# of Transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).
Note Single siRNA duplex (10nmol) can be ordered.
Reference Data
RefSeq NM_010495, NM_001355113, NM_001369018
Synonyms AI323524; bHLHb24; D2Wsu140e; Idb1
Performance Guaranteed OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency.

For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required).
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Documents
Resources

file-alt Citations

Id4 promotes the elimination of the pro-activation factor Ascl1 to maintain quiescence of adult hippocampal stem cells
Blomfield, Rocamonde, Masdeu et al
Elife (2019) 8 DOI: 10.7554/eLife.48561
AI Summary: In this study, OriGene's HPRT-targeting siRNA was used as a positive control to validate the efficiency of siRNA-mediated gene silencing in neural stem cell experiments investigating the role of Id4 in maintaining stem cell quiescence.
Snippet: … NSCs were transfected with a cocktail of three siRNAs at 20 nM each, or 60 nM control scrambled siRNA, or 60 nM HPRT-targeting siRNA as a positive control (Origene). To silence Id4 in combination with Id1, Id2 or Id3, 30 nM of Id4 ‘A’ siRNA was co-transfected with 30 nM of siRNA ‘A’ targeting Id1, 2 or 3. …
FigureFigureFigure
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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