Usp34 Mouse siRNA Oligo Duplex (Locus ID 17847)
$518.00
In Stock*
Specifications
| Product Data | |
| Purity | HPLC purified |
|---|---|
| Quality Control | Tested by ESI-MS |
| Sequences | Available with shipment |
| Components |
Usp34 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 17847)Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmolIncluded - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml
|
| Stability | One year from date of shipment when stored at -20°C. |
| Shipping | Ambient |
| # of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
| Note | Single siRNA duplex (10nmol) can be ordered. |
| Reference Data | |
| RefSeq | NM_001190401 |
| Synonyms | A530081C03Rik; Murr2 |
| Summary | Ubiquitin hydrolase that can remove conjugated ubiquitin from AXIN1 and AXIN2, thereby acting as a regulator of Wnt signaling pathway. Acts as an activator of the Wnt signaling pathway downstream of the beta-catenin destruction complex by deubiquitinating and stabilizing AXIN1 and AXIN2, leading to promote nuclear accumulation of AXIN1 and AXIN2 and positively regulate beta-catenin (CTNBB1)-mediated transcription. Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins.UniProtKB/Swiss-Prot Function |
| Performance Guaranteed | OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency. For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required). |
Reviews
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| FAQs |
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Resources
Citations
Inhibition of ubiquitin-specific protease 34 (USP34) induces epithelial-mesenchymal transition and promotes stemness in mammary epithelial cells
Oh, Kim, Sung et al
Cell. Signal. (2017) 36, 230-239 DOI: 10.1016/j.cellsig.2017.05.009
Oh, Kim, Sung et al
Cell. Signal. (2017) 36, 230-239 DOI: 10.1016/j.cellsig.2017.05.009
AI Summary: OriGene’s si-USP34 and control si-RNA products were used to knock down USP34 expression in NMuMG cells, enabling the study of USP34’s role in epithelial-mesenchymal transition and stemness in mammary epithelial cells.
Snippet: Cells were seeded into 6-well plates 24 h prior to transfection. NMuMG cells were transfected with si-USP34 (Origene, Rockville, USA) or control si-RNA (30 nM) using Lipofectamine 2000 (Invitrogen) for 24 h according to the manufacturer's instructions. ... All 2 versions Cite SaveSaving
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