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E2F1 Human siRNA Oligo Duplex (Locus ID 1869)

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SKU
SR319977
E2F1 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
  • SR319977 is the updated version of SR301310.
  $518.00
3 Weeks*

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Other products for "E2F1 or NM_005225"

Specifications
Specifications
Product Data
Purity HPLC purified
Quality Control Tested by ESI-MS
Sequences Available with shipment
Components
E2F1 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 1869)
Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmol
Included - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml
Stability One year from date of shipment when stored at -20°C.
Shipping Ambient
# of Transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).
Note Single siRNA duplex (10nmol) can be ordered.
Reference Data
RefSeq NM_005225
UniProt ID Q01094
Synonyms E2F-1; RBAP1; RBBP3; RBP3
Summary The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. provided by RefSeq, Jul 2008
Performance Guaranteed OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency.

For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required).
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Documents
Resources

file-alt Citations

E2F1 activates MFN2 expression by binding to the promoter and decreases mitochondrial fission and mitophagy in HeLa cells
Bucha, Mukhopadhyay, Bhattacharyya
FEBS J. (2019)
AI Summary: OriGene products contributed to this study by providing the E2F1 siRNA (SR301310) for gene knockdown experiments and mock siRNA (SR30004) as a control, enabling the authors to investigate the role of E2F1 in regulating MFN2 expression and mitochondrial dynamics.
Snippet: For E2F1 siRNA mediated knockdown, pooled E2F1 siRNA (Origene, SR301310) was used at a concentration of 10nM for 72h. Mock siRNA (Origene, SR30004) was used as control at the same concentration. Page 16. Accepted Article
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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