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CDC42 Human siRNA Oligo Duplex (Locus ID 998)

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SKU
SR319646
CDC42 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
  • SR319646 is the updated version of SR300714.
  $518.00
In Stock*

Frequently Bought Together (3)

TT320001 siTran 2.0 siRNA transfection reagent (0.5ml)
500 ul
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SR30002 Trilencer-27 Fluorescent-labeled transfection control siRNA duplex - 1 nmol
1 nmol
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TT300007S A non-viral, non-electroporation, non-liposomal transfection kit using engineered mammalian proteins, tailored for high-efficiency delivery into a wide range of primary cells and difficult to transfect cell lines such as immune cells, stem cells, and neuronal cells.
20 ul
$265.00

Other products for "CDC42 or NM_001039802"

Specifications
Specifications
Product Data
Purity HPLC purified
Quality Control Tested by ESI-MS
Sequences Available with shipment
Components
CDC42 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 998)
Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmol
Included - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml
Stability One year from date of shipment when stored at -20°C.
Shipping Ambient
# of Transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).
Note Single siRNA duplex (10nmol) can be ordered.
Reference Data
RefSeq NM_001039802, NM_001791, NM_044472
UniProt ID P60953
Synonyms CDC42Hs; G25K; TKS
Summary The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20. provided by RefSeq, Apr 2013
Performance Guaranteed OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency.

For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required).
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Documents
Resources

file-alt Citations

Cholesterol Efflux-Independent Modification of Lipid Rafts by AIBP (Apolipoprotein A-I Binding Protein)
Low, Mukhamedova, Capettini et al
Arterioscler. Thromb. Vasc. Biol. (2020)
AI Summary: OriGene's siRNAscram and siR-NACdc42 products were used to silence gene expression in THP-1 cells, enabling the study of how Cdc42 silencing affects AIBP-mediated regulation of lipid rafts.
Snippet: software. Protein Expression Silencing THP-1 cells were silenced using siRNAscram (Origene) or siR- NACdc42 (Origene) using INTERFERin (Polyplus Transfections) according to manufacturer's protocol with minor modifica- tions
Modulation of the Pol II CTD Phosphorylation Code by Rac1 and Cdc42 Small GTPases in Cultured Human Cancer Cells and Its Implication for Developing a Synthetic-Lethal Cancer Therapy
Zhang, Zhong, Sauane et al
Cells (2020) 9 (3) DOI: 10.3390/cells9030621
Cited by 9
AI Summary: In this study, OriGene Technologies provided the Cdc42 and Rac1 siRNA duplexes (specifically, OriGene SR300714), which were used to knock down these genes in HeLa cells and investigate their roles in modulating RNA polymerase II CTD phosphorylation in cancer cells.
Snippet: Page 3. Cells 2020, 9, 621 3 of 18 2.2. siRNA Transfection Cdc42 and Rac1 siRNA were designed and supplied by OriGene Technologies and rGrGrArGrArArCrCrA- rUrArUrArCrUrCrUrUrGrGrArCrUTT) siRNA duplexes (OriGene, SR300714) was used
Glucose insult elicits hyperactivation of cancer stem cells through miR-424-cdc42-prdm14 signalling axis
Nandy, Orozco, Lopez-Valdez et al
Br. J. Cancer (2017) 117 (11), 1665-1675 DOI: 10.1038/bjc.2017.335
AI Summary: OriGene products, specifically the cdc42 siRNA (cat. no. SR300714) and cdc42 overexpression plasmid (cat. no. RC214076), were used to modulate cdc42 expression in breast cancer cells, enabling the authors to investigate the role of cdc42 in cancer stem cell activity and invasion under different glucose conditions.
Snippet: A11138-03;Invitrogen, Carlsbad, CA, USA). MDA-MB-231 were also transfected with short interfering RNA (siRNA) for cdc42 (cat. no. SR300714;Origene, Rockville, MD, USA) and plasmid vector harbouring open reading frame for cdc42 (cat. no. RC214076;Origene)
FigureFigureFigure
KLF5 promotes breast cancer proliferation, migration and invasion in part by upregulating the transcription of TNFAIP2
Jia, Zhou, Liang et al
Oncogene (2015)
Cited by 212
AI Summary: OriGene’s pCMV6-XL5 vector containing the full-length TNFAIP2 gene (SC128195) was used as the template to generate constructs for functional studies investigating the role of TNFAIP2 in breast cancer proliferation, migration, and invasion.
Snippet: 47 To generate the pBabe-TNFAIP2 plasmid, we amplified the full-length TNFAIP2 gene coding sequence from the pCMV6-XL5 vector (SC128195;Origene, Rockville, MD, USA) and subcloned it into the pBabe-puro retroviral vector. ... Cite SaveSaving
Showing 1-4 of 4 papers.
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