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CD9 Human siRNA Oligo Duplex (Locus ID 928)

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SKU
SR319596
CD9 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
  • SR319596 is the updated version of SR300656.
  $518.00
In Stock*

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Other products for "CD9 or NM_001769"

Specifications
Specifications
Product Data
Purity HPLC purified
Quality Control Tested by ESI-MS
Sequences Available with shipment
Components
CD9 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 928)
Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmol
Included - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml
Stability One year from date of shipment when stored at -20°C.
Shipping Ambient
# of Transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).
Note Single siRNA duplex (10nmol) can be ordered.
Reference Data
RefSeq NM_001769, NM_001330312
UniProt ID P21926
Synonyms BTCC-1; DRAP-27; MIC3; MRP-1; TSPAN-29; TSPAN29
Summary This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis. provided by RefSeq, Jan 2011
Performance Guaranteed OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency.

For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required).
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Documents
Resources

file-alt Citations

Proteomic landscape of tunneling nanotubes reveals CD9 and CD81 tetraspanins as key regulators
Notario Manzano, Chaze, Rubinstein et al
Elife (2024) 13 DOI: 10.7554/eLife.99172
AI Summary: OriGene's non-targeting control siRNA was used as a negative control in experiments assessing the effects of CD9 knockdown on tunneling nanotube formation and function.
Snippet: A) Representative confocal micrograph of the cocultures between KD control cells (CTR), or the KD of CD9 (CD9 KD). Human siRNA for CD9 (sequence: GAGCATCTTCGAGCAAGAA) or non-targeting siRNA (CTR from Origene) were transiently transfected into cells using Lipofectamine RNAimax (Invitrogen), in accordance with the manufacturer’s instructions. Experiments were performed 48 hr following transfection
FigureFigureFigure
Quantitatively mapping the assembly pattern of EpCAM on cell membranes with peptide probes
Jing, Zhou, Chen et al
Anal. Chem. (2019) 92 (2), 1865-1873 DOI: 10.1021/acs.analchem.9b03901
Cited by 47
AI Summary: In this study, OriGene's siRNAs and siTran 1.0 reagent were used to knock down CD9 expression in MCF-7 cells, enabling the investigation of CD9's role in EpCAM clustering on cell membranes.
Snippet: siRNA Knockdown of CD9. MCF-7 cells were seeded on pre-cleaned coverslips (22 mm × 22 mm, Fisher) to attain a ~50% confluency. The siRNAs (OriGene) were firstly resuspended in siTran 1.0 reagent (OriGene) and phenol red- free MEM
Showing 1-2 of 2 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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