eNOS (NOS3) Human siRNA Oligo Duplex (Locus ID 4846)
$518.00
In Stock*
Specifications
| Product Data | |
| Purity | HPLC purified |
|---|---|
| Quality Control | Tested by ESI-MS |
| Sequences | Available with shipment |
| Components |
NOS3 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 4846)Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmolIncluded - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml
|
| Stability | One year from date of shipment when stored at -20°C. |
| Shipping | Ambient |
| # of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
| Note | Single siRNA duplex (10nmol) can be ordered. |
| Reference Data | |
| RefSeq | NM_000603, NM_001160109, NM_001160110, NM_001160111 |
| UniProt ID | P29474 |
| Synonyms | ECNOS; eNOS |
| Summary | Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. provided by RefSeq, Oct 2016 |
| Performance Guaranteed | OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency. For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required). |
Reviews
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| FAQs |
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Resources
Citations
MALAT1 and HOTAIR Long Non-Coding RNAs Play Opposite Role in Estrogen-Mediated Transcriptional Regulation in Prostate Cancer Cells
Aiello, Bacci, Re et al
Sci Rep (2016) 6, 38414 DOI: 10.1038/srep38414
Aiello, Bacci, Re et al
Sci Rep (2016) 6, 38414 DOI: 10.1038/srep38414
AI Summary: OriGene’s Trilencer-27mer siRNA duplexes were used to specifically silence eNOS expression in prostate cancer cells, enabling the study of how eNOS influences estrogen-mediated transcriptional regulation involving MALAT1 and HOTAIR lncRNAs.
Snippet: Small interference to eNOS was obtained with 100 nM Trilencer-27mer siRNA duplexes (Origene, Rockville, MD) transfected by Lipofectamine RNAiMAX according to the manufacturer’s instructions in hormone-deprived serum and after 72 h treated whit 17β-estradiol (E2)
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
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