Atg5 Rat shRNA Plasmid (Locus ID 365601)
SKU
TR702366
Atg5 - Rat, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
$883.00
In Stock*
Specifications
| Product Data | |
| Locus ID | 365601 |
|---|---|
| Vector | pRS |
| E. coli Selection | Ampicillin |
| Mammalian Cell Selection | Puromycin |
| Format | Retroviral plasmids |
| Components | Atg5 - Rat, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 365601). 5µg purified plasmid DNA per construct29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free. |
| RefSeq | NM_001014250, NM_001014250.1, BC083602 |
| shRNA Design | These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service. |
| Storage | Store at -20°C |
| Shipping | Ambient |
Reviews
Documents
Resources
Citations
Chloroquine reverses chemoresistance via upregulation of p21WAF1/CIP1 and autophagy inhibition in ovarian cancer
Hwang, Kim, Cho et al
Cell Death Dis (2020) 11 (12), 1034 DOI: 10.1038/s41419-020-03242-x
Hwang, Kim, Cho et al
Cell Death Dis (2020) 11 (12), 1034 DOI: 10.1038/s41419-020-03242-x
Cited by 64
AI Summary: OriGene’s p21WAF1/CIP1-specific shRNA and GFP-expressing scrambled shRNA were used to knock down gene expression in ovarian cancer cells, enabling the study to demonstrate the role of p21WAF1/CIP1 in chloroquine-mediated reversal of chemoresistance.
Snippet: ll lines To prepare lentiviruses expressing shRNA, plasmids for lentivirus preparation, pMD.2G (Addgene, MA, USA) and psPAX2 (Addgene), and plasmid encoding either ATG5-, p21WAF1/CIP1-specific shRNA (Origene, MD, USA), or GFP-expressing scrambled shRNA (Origene) were transfected into 293T cells using Lipofectamine 2000 (Invitrogen). Lentivirus expressing shRNA was prepared as previously described3
Showing 1-1 of 1 papers.
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