Kmt2a Mouse shRNA Plasmid (Locus ID 214162)
SKU
TR517798
Kmt2a - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
$883.00
In Stock*
Specifications
| Product Data | |
| Locus ID | 214162 |
|---|---|
| Synonyms | 6430520K01; ALL-1; All1; Cxxc7; HRX; HTRX1; mKIAA4050; Mll; Mll1 |
| Vector | pRS |
| E. coli Selection | Ampicillin |
| Mammalian Cell Selection | Puromycin |
| Format | Retroviral plasmids |
| Components | Kmt2a - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector (Gene ID = 214162). 5µg purified plasmid DNA per construct29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free. |
| RefSeq | NM_001081049, NM_001357549, BC044818 |
| Summary | Histone methyltransferase that plays an essential role in early development and hematopoiesis. Catalytic subunit of the MLL1/MLL complex, a multiprotein complex that mediates both methylation of 'Lys-4' of histone H3 (H3K4me) complex and acetylation of 'Lys-16' of histone H4 (H4K16ac). In the MLL1/MLL complex, it specifically mediates H3K4me, a specific tag for epigenetic transcriptional activation. Has weak methyltransferase activity by itself, and requires other component of the MLL1/MLL complex to obtain full methyltransferase activity. Has no activity toward histone H3 phosphorylated on 'Thr-3', less activity toward H3 dimethylated on 'Arg-8' or 'Lys-9', while it has higher activity toward H3 acetylated on 'Lys-9'. Binds to unmethylated CpG elements in the promoter of target genes and helps maintain them in the nonmethylated state. Required for transcriptional activation of HOXA9 (By similarity). Promotes PPP1R15A-induced apoptosis. Plays a critical role in the control of circadian gene expression and is essential for the transcriptional activation mediated by the CLOCK-ARNTL/BMAL1 heterodimer. Establishes a permissive chromatin state for circadian transcription by mediating a rhythmic methylation of 'Lys-4' of histone H3 (H3K4me) and this histone modification directs the circadian acetylation at H3K9 and H3K14 allowing the recruitment of CLOCK-ARNTL/BMAL1 to chromatin (PubMed:21113167).UniProtKB/Swiss-Prot Function |
| shRNA Design | These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service. |
| Storage | Store at -20°C |
| Shipping | Ambient |
Reviews
Documents
Resources
Citations
Protein kinase Msk1 physically and functionally interacts with the KMT2A/MLL1 methyltransferase complex and contributes to the regulation of multiple target genes
Wiersma, Bussiere, Halsall et al
Epigenetics Chromatin (2016) 9, 52 DOI: 10.1186/s13072-016-0103-3
Wiersma, Bussiere, Halsall et al
Epigenetics Chromatin (2016) 9, 52 DOI: 10.1186/s13072-016-0103-3
Cited by 32
AI Summary: OriGene’s shRNA vectors (Msk1: TR504795; MLL1: TR517798) were used to knock down Msk1 and MLL1 expression in mouse embryonic fibroblasts, enabling the study of their roles in gene regulation.
Snippet: for 30 min, 4 °C with rotation and the supernatants pooled. shRNA knockdowns shRNA vectors were purchased from Origene (Msk1: TR504795;MLL1: TR517798) and transfected into MEFs by electroporation (GenePulser X
Showing 1-1 of 1 papers.
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