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Foxk2 Mouse shRNA Plasmid (Locus ID 68837)

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SKU
TR517739
Foxk2 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
  $883.00
2 Weeks*

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Specifications
Specifications
Product Data
Locus ID 68837
Synonyms 1110054H05Rik; 5730434B08Rik; 6230415M23Rik; ILF; Ilf1
Vector pRS
E. coli Selection Ampicillin
Mammalian Cell Selection Puromycin
Format Retroviral plasmids
Components Foxk2 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 68837). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq NM_001080932, NM_001080932.1, NM_001080932.2, BC033431, BC045204, BC072609, BC152767, NM_001363033, NM_001080932.3
UniProt ID Q3UCQ1
Summary Transcriptional regulator involved in different processes such as glucose metabolism, aerobic glycolysis and autophagy (PubMed:25402684, PubMed:29861159, PubMed:30700909). Recognizes and binds the forkhead DNA sequence motif (5'-GTAAACA-3') and can both act as a transcription activator or repressor, depending on the context (PubMed:25402684, PubMed:29861159, PubMed:30700909). Together with FOXK1, acts as a key regulator of metabolic reprogramming towards aerobic glycolysis, a process in which glucose is converted to lactate in the presence of oxygen (PubMed:30700909). Acts by promoting expression of enzymes for glycolysis (such as hexokinase-2 (HK2), phosphofructokinase, pyruvate kinase (PKLR) and lactate dehydrogenase), while suppressing further oxidation of pyruvate in the mitochondria by up-regulating pyruvate dehydrogenase kinases PDK1 and PDK4 (PubMed:30700909). Probably plays a role in gluconeogenesis during overnight fasting, when lactate from white adipose tissue and muscle is the main substrate (PubMed:30700909). Together with FOXK1, acts as a negative regulator of autophagy in skeletal muscle: in response to starvation, enters the nucleus, binds the promoters of autophagy genes and represses their expression, preventing proteolysis of skeletal muscle proteins (PubMed:25402684). In addition to the 5'-GTAAACA-3' DNA motif, also binds the 5'-TGANTCA-3' palindromic DNA motif, and co-associates with JUN/AP-1 to activate transcription (By similarity). Also able to bind to a minimal DNA heteroduplex containing a G/T-mismatch with 5'-TRTG/TNB-3' sequence (By similarity). Binds to NFAT-like motifs (purine-rich) in the IL2 promoter (By similarity). Positively regulates WNT/beta-catenin signaling by translocating DVL proteins into the nucleus (By similarity).UniProtKB/Swiss-Prot Function
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Storage Store at -20°C
Shipping Ambient
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Documents
Resources

file-alt Citations

FOXK1 and FOXK2 regulate aerobic glycolysis
Sukonina, Ma, Zhang et al
Nature (2019) 566 (7743), 279-283 DOI: 10.1038/s41586-019-0900-5
AI Summary: OriGene’s pRS vector was used to clone shRNAs for stable knockdown of mouse Foxk1 and Foxk2, enabling the authors to study the functional effects of reducing these genes on cellular metabolism.
Snippet: Full-length human FOXK1 (NM_001037165.1) and FOXK2 (NM_004514.3) with C-terminal triple Flag-tag (3×Flag) were created in pcDNA3.1/Hygro(+)vector and subcloned into retroviral pBabe-puro, pRetroXTRE3G and lentiviral p156RRLsinPPTCAG-PRE or pLVXpuro vectors for constitutive or doxycycline-inducible expression of proteins. For stable knockdown of mouse Foxk1 and Foxk2, two independent shRNAs for each gene were cloned into a retroviral pRS vector (OriGene)
Showing 1-1 of 1 papers.
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