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Pfkfb3 Mouse shRNA Plasmid (Locus ID 170768)

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SKU
TR517196
Pfkfb3 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
  $883.00
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Specifications
Specifications
Product Data
Locus ID 170768
Synonyms E330010H22Rik; iPFK-2; uPFK-2
Vector pRS
E. coli Selection Ampicillin
Mammalian Cell Selection Puromycin
Format Retroviral plasmids
Components Pfkfb3 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 170768). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq BC052400, BC057320, NM_001177752, NM_001177753, NM_001177754, NM_001177755, NM_001177756, NM_001177757, NM_001177758, NM_133232, NM_001177757.1, NM_001177752.1, NM_001177753.1, NM_001177754.1, NM_001177755.1, NM_001177756.1, NM_133232.1, NM_133232.2, NM_133232.3, BC043926, NM_172976
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Storage Store at -20°C
Shipping Ambient
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Documents
Resources

file-alt Citations

A ROLE FOR PFKFB3/IPFK2 IN OVERNUTRITION-ASSOCIATED ADIPOSE TISSUE AND INTESTINE INFLAMMATORY RESPONSES AND INSULIN RESISTANCE
Guo, Huo, Chen et al
Thesis (2013)
AI Summary: OriGene’s shRNA plasmid targeting mouse PFKFB3 was used to knock down PFKFB3/iPFK2 expression in 3T3-L1 cells, enabling the study of its role in overnutrition-associated inflammation and insulin resistance.
Snippet: medium supplemented with 10 g/ml insulin. To knock down PFKFB3/iPFK2, predifferentiated 3T3-L1 cells were transfected with the plasmid containing shRNA against mouse PFKFB3 (iPFK2-KD) (OriGene, Rockville, MD)
Disruption of inducible 6-phosphofructo-2-kinase ameliorates diet-induced adiposity but exacerbates systemic insulin resistance and adipose tissue inflammatory response
Huo, Guo, Li et al
J Biol Chem (2010) 285 (6), 3713-3721 DOI: 10.1074/jbc.M109.058446
AI Summary: In this study, OriGene’s PFKFB3 shRNA (OriGene, catalog # TG501513) was used to knock down PFKFB3/iPFK2 expression in 3T3-L1 adipocytes, enabling the authors to investigate the effects of reduced PFKFB3/iPFK2 on glucose metabolism, insulin signaling, and inflammatory responses.
Showing 1-2 of 2 papers.
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