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Nlrp3 Mouse shRNA Plasmid (Locus ID 216799)

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SKU
TR510752
Nlrp3 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
  $883.00
2 Weeks*

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Specifications
Specifications
Product Data
Locus ID 216799
Synonyms AGTAVPRL; AII/AVP; Cias1; FCAS; FCU; Mmig1; MWS; NALP3; Pypaf1
Vector pRS
E. coli Selection Ampicillin
Mammalian Cell Selection Puromycin
Format Retroviral plasmids
Components Nlrp3 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 216799). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq BC116174, BC116175, NM_145827, NM_001359638, NR_153314, NM_145827.1, NM_145827.2, NM_145827.3
UniProt ID Q8R4B8
Summary As the sensor component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens and other damage-associated signals, initiates the formation of the inflammasome polymeric complex, made of NLRP3, PYCARD and CASP1 (or possibly CASP4/CASP11). Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and secretion in the extracellular milieu (PubMed:28847925). Activation of NLRP3 inflammasome is also required for HMGB1 secretion (PubMed:22801494). The active cytokines and HMGB1 stimulate inflammatory responses. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death. Under resting conditions, NLRP3 is autoinhibited. NLRP3 activation stimuli include extracellular ATP, reactive oxygen species, K(+) efflux, crystals of monosodium urate or cholesterol, amyloid-beta fibers, environmental or industrial particles and nanoparticles, cytosolic dsRNA, etc. However, it is unclear what constitutes the direct NLRP3 activator. Activation in presence of cytosolic dsRNA is mediated by DHX33 (By similarity). Independently of inflammasome activation, regulates the differentiation of T helper 2 (Th2) cells and has a role in Th2 cell-dependent asthma and tumor growth. During Th2 differentiation, required for optimal IRF4 binding to IL4 promoter and for IRF4-dependent IL4 transcription. Binds to the consensus DNA sequence 5'-GRRGGNRGAG-3'. May also participate in the transcription of IL5, IL13, GATA3, CCR3, CCR4 and MAF (PubMed:26098997).UniProtKB/Swiss-Prot Function
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Storage Store at -20°C
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Documents
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file-alt Citations

Procyanidin B2 protects rats from paraquat-induced acute lung injury by inhibiting NLRP3 inflammasome activation
Jiang, Yang, Gui
Immunobiology (2018) 223 (10), 555-561 DOI: 10.1016/j.imbio.2018.07.001
AI Summary: OriGene products contributed to this study by providing NLRP3 shRNA and scrambled shRNA plasmids, which were used to silence the NLRP3 gene and assess its role in paraquat-induced lung injury in rats.
Snippet: 2.8. NLRP3 Gene silencing pretreatment. Two days before paraquat administration, using the sequences described by (Wu et al., 2015), NLRP3 siRNA and scrambled siRNA were used to construct NLRP3 shRNA and scrambled shRNA plasmids (OriGene Technologies
Contribution of redox-dependent activation of endothelial Nlrp3 inflammasomes to hyperglycemia-induced endothelial dysfunction
Chen, Wang, Pitzer et al
J. Mol. Med. (2016)
AI Summary: In this study, the Nlrp3 shRNA plasmid from OriGene was used to silence the Nlrp3 gene in mouse vascular endothelial cells, enabling the researchers to investigate the role of Nlrp3 inflammasome activation in hyperglycemia-induced endothelial dysfunction.
Snippet: Transfection of shRNA plasmids was performed using a 4D Nucleofector X-Unit(Lonza, CA, USA) according to the manufacturer's instructions [26]. The Nlrp3 shRNA plasmidwas obtained from Origene. Briefly, MVECs were tyrpsinized and centrifuged at 80g for 10 ... Cite SaveSaving
Coronary endothelial dysfunction induced by nucleotide oligomerization domain-like receptor protein with pyrin domain containing 3 inflammasome activation during hypercholesterolemia: beyond inflammation
Zhang, Li, Pitzer et al
Antioxid Redox Signal (2015) 22 (13), 1084-96 DOI: 10.1089/ars.2014.5978
Cited by 91
AI Summary: In this study, OriGene products—specifically the Nlrp3 knockout (Nlrp3(-/-)) mice—were used to investigate the role of the Nlrp3 inflammasome in coronary endothelial dysfunction during hypercholesterolemia.
Gene silencing of NLRP3 protects against the sepsis-induced hyper -bileacidemia in a rat model
Wu, Ren, Zhou et al
Clin. Exp. Immunol. (2014)
Cited by 37
AI Summary: OriGene contributed NLRP3 shRNA and scrambled shRNA plasmids, which were used to selectively silence the NLRP3 gene and investigate its role in sepsis-induced hyper-bileacidemia in rats.
Snippet: Materials and reagents Sprague-Dawley rats (animal model institute of Xi'an, China);NLRP3 shRNA and scrambled shRNA plasmids (Origene, USA);anti-NLRP3 antibody (Abcam, ... construct NLRP3 shRNA and scrambled shRNA plasmids (Origene, USA). The ... Cite SaveSaving
Showing 1-4 of 4 papers.
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