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P4hb Mouse shRNA Plasmid (Locus ID 18453)

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SKU
TR501569
P4hb - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
  $883.00
In Stock*

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Specifications
Specifications
Product Data
Locus ID 18453
Synonyms ERp59; PDI; Pdia1; Thbp
Vector pRS
E. coli Selection Ampicillin
Mammalian Cell Selection Puromycin
Format Retroviral plasmids
Components P4hb - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 18453). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq BC008549, BC093512, NM_011032, NM_011032.1, NM_011032.2, BC018581
UniProt ID P09103
Summary This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP (By similarity). Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration (PubMed:21670307).UniProtKB/Swiss-Prot Function
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Storage Store at -20°C
Shipping Ambient
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Documents
Resources

file-alt Citations

The protein disulfide isomerase P4HB/PDIA1 modulates cellular and misfolded forms of the prion protein
Amano, Arshad, Patel et al
PLoS Pathog (2026) 22 (8), e1014536 DOI: 10.1371/journal.ppat.1014536
AI Summary: OriGene’s P4HB shRNA (catalog #TR501569) was used in this study to knock down P4HB expression in CAD5 cells, enabling the authors to investigate the role of P4HB in prion protein regulation and misfolding.
Snippet: ted at a density of 4.0 x 105 cells in 6-cm dishes. Prion-infected CAD5 cells were plated at 6.0 x 105 cells in 6-cm dishes. The following day, 6.0 µg of control or P4HB shRNA (ORIGENE #TR501569) was mixed with 12 µL of Lipofectamine 2000 in 500 µL of serum-free Opti-MEM medium, and the mixture was added to the cells and incubated for 24 h. After 24 h post-transfection, th
FigureFigureFigure
The Protein Disulfide Isomerase P4HB/PDIA1 Modulates PrPC Levels and Prion Replication
Amano, Arshad, Patel et al
bioRxiv (2025)
AI Summary: OriGene’s P4HB shRNA (OriGene #TR501569) was used to stably knock down P4HB expression in CAD5 cells, enabling the study of its role in regulating PrPC levels and prion replication.
Snippet: For generation of CAD5 cells stably transfected with P4HB shRNA, CAD5 cells were seeded at a density 5.0 x 105 cells/well in a 6-well plate. The following day, 2 µg of control or P4HB shRNA (ORIGENE #TR501569) was mixed with 4 µL of Lipofectamine 2000 in 200 µL of serum-free Opti-MEM medium, and the mixture was added to the cells and incubated for 24 h. A
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