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Atg5 Mouse shRNA Plasmid (Locus ID 11793)

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SKU
TR500113
Atg5 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
  $883.00
In Stock*

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Specifications
Specifications
Product Data
Locus ID 11793
Synonyms 2010107M05Rik; 3110067M24Rik; Ap; Apg5l; Atg5l; AW319544; C88337; Pad; Paddy
Vector pRS
E. coli Selection Ampicillin
Mammalian Cell Selection Puromycin
Format Retroviral plasmids
Components Atg5 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 11793). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq BC002166, NM_053069, NM_053069.1, NM_053069.2, NM_053069.3, NM_053069.4, NM_053069.5, NM_053069.6
UniProt ID Q99J83
Summary The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Two transcript variants encoding different protein isoforms have been found for this gene. provided by RefSeq, Sep 2015
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Storage Store at -20°C
Shipping Ambient
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Documents
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file-alt Citations

Autophagic clearance of mitochondria in the kidney copes with metabolic acidosis
Namba, Takabatake, Kimura et al
J Am Soc Nephrol (2014) 25 (10), 2254-66 DOI: 10.1681/ASN.2013090986
AI Summary: In this paper, OriGene’s pCMV6-Entry-ATG5 plasmid was used to restore ATG5 expression in Atg5-deficient proximal tubular cells, enabling the authors to demonstrate that reconstitution of autophagy rescues mitochondrial function and ammoniagenesis under metabolic acidosis conditions.
Immunohistochemical detection of cytoplasmic LC3 puncta in human cancer specimens
Ladoire, Chaba, Martins et al
Autophagy (2012) 8 (8), 1175-84 DOI: 10.4161/auto.20353
Cited by 79
AI Summary: OriGene products, specifically the shRNA plasmids targeting murine Atg5 (TR500113) and Atg7 (TR504956), were used to generate autophagy-deficient cancer cells for validating the immunohistochemical detection of LC3 puncta in the study.
Snippet: To obtain autophagy-deficient CT26 and MCA205 cells, a set of plasmids encoding short-hairpin RNAs (shRNAs) specific for murine Atg5 (TR500113) and Atg7 (TR504956) plus a control shRNA were obtained from OriGene. These plasmids were used
ATG7 deficiency suppresses apoptosis and cell death induced by lysosomal photodamage
Kessel, Price, Reiners
Autophagy (2012) 8 (9), 1333-41 DOI: 10.4161/auto.20792
Cited by 110
AI Summary: OriGene’s pRS plasmids expressing murine Atg5 shRNA (TR500113) were used to generate ATG5-deficient 1c1c7 cells, enabling the study of autophagy’s role in cell death and apoptosis following lysosomal photodamage.
Snippet: 11 1c1c7 cells that stably express shRNA to murine Atg5 (ie, 1c1c7 ATG5 KD) were prepared by transfection with a mixture of 4 pRS plasmids that each express a different murine Atg5 shRNA (OriGene, TR500113), followed by two weeks of selection in growth medium
Showing 1-3 of 3 papers.
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