BCDIN3D Human shRNA Plasmid Kit (Locus ID 144233)
SKU
TR317908
BCDIN3D - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
$883.00
2 Weeks*
Specifications
| Product Data | |
| Locus ID | 144233 |
|---|---|
| Vector | pRS |
| E. coli Selection | Ampicillin |
| Mammalian Cell Selection | Puromycin |
| Format | Retroviral plasmids |
| Components | BCDIN3D - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 144233). 5µg purified plasmid DNA per construct29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free. |
| RefSeq | NM_181708, NM_181708.1, NM_181708.2, BC053560, BC053560.1, BC040957, NM_181708.3 |
| UniProt ID | Q7Z5W3 |
| Summary | This gene encodes an RNA methyltransferase which belongs to the rossmann fold methyltransferase family, and serves as a 5'-methylphosphate capping enzyme that is specific for cytoplasmic histidyl tRNA. The encoded protein contains an S-adenosylmethionine binding domain and uses the methyl group donor, S-adenosylmethionine. This gene is overexpressed in breast cancer cells, and is related to the tumorigenic phenotype and poor prognosis of breast cancer. The encoded protein is thought to promote the cellular invasion of breast cancer cells, by downregulating the expression of tumor suppressor miRNAs through the dimethylation of the 5-monophosphate of the corresponding precursor miRNAs. provided by RefSeq, Apr 2017 |
| shRNA Design | These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service. |
| Storage | Store at -20°C |
| Shipping | Ambient |
Reviews
Documents
Resources
Citations
BCDIN3D regulates tRNAHis 3' fragment processing
Reinsborough, Ipas, Abell et al
PLoS Genet. (2019) 15 (7), e1008273 DOI: 10.1371/journal.pgen.1008273
Reinsborough, Ipas, Abell et al
PLoS Genet. (2019) 15 (7), e1008273 DOI: 10.1371/journal.pgen.1008273
AI Summary: OriGene’s pRS-shBCDIN3D TR317908C/TI368844 plasmid and pRS-Scrambled TR30012 control plasmid were used to generate BCDIN3D knockdown and control cell lines, enabling the study of BCDIN3D’s role in tRNAHis 3'-fragment processing.
Snippet: ted with 200 μg/ml of Zeocin (parental) or 400 μg/ml hygromycin (BCDIN3Df). MDA-MB-231shBCDIN3D were generated by transfection of MDA-MB-231 cells with pRS-shBCDIN3D TR317908C/TI368844 plasmid from Origene, while the matched MDA-MB-231shNC were generated by transfection with pRS-Scrambled TR30012. These cells were grown in DMEM+10%FBS+PSQ+1 μg/ml of puromycin. The Hap1 Parental and BCDIN3D KO c
Human RNA methyltransferase BCDIN3D regulates microRNA processing
Xhemalce, Robson, Kouzarides
Cell (2012) 151 (2), 278-88 DOI: 10.1016/j.cell.2012.08.041
Xhemalce, Robson, Kouzarides
Cell (2012) 151 (2), 278-88 DOI: 10.1016/j.cell.2012.08.041
Cited by 152
AI Summary: OriGene’s pMirTarget vector was used to assess the regulatory effects of BCDIN3D on miRNA-mediated gene expression by fusing the 3′-UTR of IRS1 to a luciferase reporter in transfected cells.
Showing 1-2 of 2 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
