CD9 Human shRNA Plasmid Kit (Locus ID 928)

Snippet: Stable knockdown of CD9 in RWPE1 cells was conducted as described above using the pRS CD9 shRNA vector (Origene Cat# TR314060;Australian Biosearch, Karrinyup, WA, Australia) and selection with 1.5 µg/mL Puromycin (Sigma Aldrich, Castle Hill, NSW, Australia) to achieve stable knockdown of CD9, herein referred to as CD9 low. …

Snippet: … Expression constructs and RNA silencing transfection For stable transfection experiments, HSB-2 and U937 cells were electroporated with 20 μg pcDNA3-CD9 plasmid at 200 V (2 × 10 ms pulses in a 0.4 cm electroporation cuvette) using an ECM830 BTX electroporation system and selected with 1 mg/ml G418. For CD9 silencing, Jurkat cells were retroviraly transduced (OriGene Technologies) with the shRNA-coding plasmids TI356235 (the plasmid with the CD9 shRNA cassette insert) and TR20003 (“TR2” control plasmid without shRNA insert), according to manufacturer's indications and selected with 1 μg/ml puromycin