EIF3F Human shRNA Plasmid Kit (Locus ID 8665)

SKU TR313251

EIF3F - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided

Product Data

• Locus ID: 8665
• Synonyms: eIF3-p47; EIF3S5; MRT67
• Vector: pRS
• E. coli Selection: Ampicillin
• Format: Retroviral plasmids
• Components: EIF3F - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 8665). 5µg purified plasmid DNA per construct; 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
• RefSeq: NM_003754, NM_003754.1, NM_003754.2, BC000490, BC000490.2, BC073753
• UniProt ID: O00303
• Summary: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).[UniProtKB/Swiss-Prot Function]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
• Shipping: Ambient