GPR43 (FFAR2) Human shRNA Plasmid Kit (Locus ID 2867)
SKU
TR313000
FFAR2 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
$883.00
In Stock*
Specifications
| Product Data | |
| Locus ID | 2867 |
|---|---|
| Synonyms | FFA2R; GPR43 |
| Vector | pRS |
| E. coli Selection | Ampicillin |
| Mammalian Cell Selection | Puromycin |
| Format | Retroviral plasmids |
| Components | FFAR2 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 2867). 5µg purified plasmid DNA per construct29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free. |
| RefSeq | NM_005306, NM_005306.1, NM_005306.2, BC095535, BC096198, BC096199, BC096200, BC096201, NM_001370087, NM_005306.3 |
| UniProt ID | O15552 |
| Summary | This gene encodes a member of the GP40 family of G protein-coupled receptors that are clustered together on chromosome 19. The encoded protein is a receptor for short chain free fatty acids and may be involved in the inflammatory response and in regulating lipid plasma levels. provided by RefSeq, Apr 2009 |
| shRNA Design | These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service. |
| Storage | Store at -20°C |
| Shipping | Ambient |
Reviews
Documents
Resources
Citations
Free fatty acids receptors 2 and 3 control cell proliferation by regulating cellular glucose uptake
Al Mahri, Al Ghamdi, Akiel et al
World J Gastrointest Oncol (2020) 12 (5), 514-525 DOI: 10.4251/wjgo.v12.i5.514
Al Mahri, Al Ghamdi, Akiel et al
World J Gastrointest Oncol (2020) 12 (5), 514-525 DOI: 10.4251/wjgo.v12.i5.514
Cited by 25
AI Summary: OriGene products, specifically FFAR2 short-hairpin RNA (shRNA) plasmids targeting four sequences of the FFAR2 gene and a control scrambled shRNA plasmid, were used to generate stable FFAR2 knockdown HCT116 cell lines for functional studies in this paper.
Snippet: Generation of stable FFAR2 KD HCT116 cell line. FFAR2 short-hairpin RNA (shRNA), targeting 4 different sequences of the FFAR2 gene, and a control scrambled shRNA plasmid were obtained from Origene Technologies, Inc;FFAR2 short-hairpin RNA (shRNA), targeting 4 different sequences of the FFAR2 gene, and a control scrambled shRNA plasmid were obtained from Origene Technologies, Inc. 1 × 105 HCT116 cells were plated in a 24-well plate and transfected with 0.5 μg of plasmid DNA using Lipofectamine3000 with a ratio of 1:2 (DNA:Lipo3000) according to the manufacturer’s protocol. … Colonies were screened for FFAR2 KD using quantitative real time polymerase chain reaction (qRT-PCR) and one colony with most efficient KD was selected for subsequent experiments. …
Showing 1-1 of 1 papers.
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