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NOLC1 Human shRNA Plasmid Kit (Locus ID 9221)

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SKU
TR311147
NOLC1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
  $883.00
2 Weeks*

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Specifications
Specifications
Product Data
Locus ID 9221
Synonyms NOPP130; NOPP140; NS5ATP13; P130; Srp40
Vector pRS
E. coli Selection Ampicillin
Mammalian Cell Selection Puromycin
Format Retroviral plasmids
Components NOLC1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 9221). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq NM_001284388, NM_001284389, NM_004741, NM_004741.1, NM_004741.2, NM_004741.3, NM_004741.4, NM_001284389.1, NM_001284388.1, BC001883, BC001883.1, BC006769, BM473772, BM849059, NM_001284389.2, NM_004741.5
UniProt ID Q14978
Summary Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification (PubMed:10567578, PubMed:26399832). Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with TCOF1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification (PubMed:26399832). Involved in nucleologenesis, possibly by playing a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus (PubMed:9016786). It has intrinsic GTPase and ATPase activities (PubMed:9016786).UniProtKB/Swiss-Prot Function
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Storage Store at -20°C
Shipping Ambient
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Documents
Resources

file-alt Citations

Tissue-specific gene targeting by the multiprotein mammalian DREAM complex
Flowers, Beck, Moran
J. Biol. Chem. (2011) 286 (32), 27867-71 DOI: 10.1074/jbc.C111.255091
Cited by 2
AI Summary: OriGene’s HuSH system was used to generate p130-depleted cell lines via RNA interference, enabling the study of DREAM complex function in tissue-specific gene repression.
Snippet: The p130-depleted lines were generated using the OriGene HuSH system and puromycin selection with the knockdown sequence ... 1, B and C). The arrested cells then lay down a collagenous extracellular matrix that undergoes mineralization beginning at about day 14.
Showing 1-1 of 1 papers.
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