SUPT5H Human shRNA Plasmid Kit (Locus ID 6829)
SKU
TR309005
SUPT5H - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
$883.00
2 Weeks*
Specifications
| Product Data | |
| Locus ID | 6829 |
|---|---|
| Synonyms | SPT5; SPT5H; Tat-CT1 |
| Vector | pRS |
| E. coli Selection | Ampicillin |
| Mammalian Cell Selection | Puromycin |
| Format | Retroviral plasmids |
| Components | SUPT5H - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 6829). 5µg purified plasmid DNA per construct29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free. |
| RefSeq | NM_001111020, NM_001130824, NM_001130825, NM_001319990, NM_001319991, NM_003169, NM_003169.1, NM_003169.2, NM_003169.3, NM_001130825.1, NM_001111020.1, NM_001111020.2, NM_001130824.1, BC024203, BC024203.2, NM_001130824.2, NM_001111020.3 |
| UniProt ID | O00267 |
| Summary | Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.UniProtKB/Swiss-Prot Function |
| shRNA Design | These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service. |
| Storage | Store at -20°C |
| Shipping | Ambient |
Reviews
Documents
Resources
Citations
A role for the RNA pol II-associated PAF complex in AID-induced immune diversification
Willmann, Milosevic, Pauklin et al
J Exp Med (2012) 209 (11), 2099-111 DOI: 10.1084/jem.20112145
Willmann, Milosevic, Pauklin et al
J Exp Med (2012) 209 (11), 2099-111 DOI: 10.1084/jem.20112145
Cited by 81
AI Summary: In this paper, OriGene's PAF1 shRNA (OriGene, #TF314515) was used to deplete PAF1 in B cells, enabling the authors to demonstrate that loss of PAF1 impairs AID-induced immune diversification and highlighting the functional importance of the PAF complex in this process.
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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