WNT6 Human shRNA Plasmid Kit (Locus ID 7475)

Snippet: … Due to its critical functions during embryogenesis 37-42, deregulated expression of WNT6 in adults may favor tumorigenesis 13-16. Our data show that the expression of WNT6 in gliomas is grade-dependent, at the gene and protein levels (Figure 1), similarly to what was previously described for other WNT ligands 5;Cells were transfected with WNT6 gene-specific shRNA expression vector inserted in a pRS plasmid (TR308360, clones TI333434 and TI333435 from Origene;named U373-shWNT6 or SNB19-shWNT6, respectively) or with a scrambled negative control non-effective shRNA in a pRS plasmid (TR30012, Origene;named U373-shCtrl or SNB19-shCtrl)