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MIR29a Rat MicroRNA Expression Plasmid (MI0000863)

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SKU
SC403263
Expression Plasmid of Rat microRNA MIR29a
  $424.00
In Stock*

Frequently Bought Together (1)

CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
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Specifications
Specifications
Product Data
Species Rat
Vector pCMV-MIR
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequences
Precursor: ACCCCUUAGAGGAUGACUGAUUUCUUUUGGUGUUCAGAGUCAAUAGAAUUUUCUAGCACCAUCUGAAAUCGGUUAUAAUGAUUGGGGA:
Mature Sequence: ACUGAUUUCUUUUGGUGUUCAG:
Full Insert
>MI0000863 TCTTGGTGGAGAACAACTTCGCTGCCGACCACACAAAAGGAAGGAGTGGACAGCCCTGAGGTATTCAGTA
TCTAAGACAGTCAGGCCACCAGAACCTGCTCAAGTAGCAGCGGGCCTGCCCTCGCCCGCCCTGCTTACCT
CGGTGTTGTGGATAGCTAATTAGTCATCCGTTGTCAGAATTTCATACTTGTTCACATAGTAAGCCTACTC
TGGAAGCAGACTCCATCAAGCCTGGATGAAGACCCTACATGTACAACAGACAGAAGGCCCGGGCCTTCCC
AATGCACATGACCTCTTGTGACCCCTTAGAGGATGACTGATTTCTTTTGGTGTTCAGAGTCAATAGAATT
TTCTAGCACCATCTGAAATCGGTTATAATGATTGGGGAAAAGCACCATGCAGCTGACTCGAGACTAATCT
ATTGTTGACTTTGGAGCCACAGGTAAGAATTAAGGAAAAAAATACTAAGACATACTAAGGCACATTTTCC
CTCTTCGTATTTTTAACTATTCACATAGTGATGGTAAATAGTGTTTTATAAAGTGTGTGGGGATTGAAGG
TTTGATGAGAACTAGAGGATAAAGGGGCAAAACCAGTGTCTGGCCAGCCAATGTGAGAGGAGCAGGAAAC
ATTCACCTCTAGAAGGTTCTATCGGCTCACCTAGCCTGCCAGGCCACGGGCCACTGCA
Components 1 x 10 ug vial miRNA plasmid lyophilized in a 2-D bar-coded Matrix tube
Also Contains Both mature and minor (star) miRNA can be over expressed from the construct.
OTI Disclaimer All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
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Reference Data
RefSeq MI0000863
Synonyms rno-mir-29a
Locus ID 100314230
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Documents
Resources

file-alt Citations

Downregulation of the human peripheral myelin protein 22 gene by miR-29a in cellular models of Charcot-Marie-Tooth disease
Serfecz, Bazick, Al Salihi et al
Gene Ther. (2019) 26 (12), 455-464 DOI: 10.1038/s41434-019-0098-z
AI Summary: OriGene products contributed to this study by providing the empty pCMV vector (CAT#: PCMVMIR), the miR-29a expression plasmid (MI0000087), and the MegaTran transfection reagent (TT200003), which were used to transfect mouse Schwann cells and assess the effect of miR-29a on PMP22 gene expression.
Snippet: Transfection of mouse Schwann cell cultures. Primary mouse Schwann cells from WT and C22 mice were transfected with 1 µg of empty pCMV vector (CAT#: PCMVMIR) or with miR-29a (ORIGENE MI0000087) for 6 h using MegaTran (ORIGENE TT200003)
Altered miRNA-29 Expression in Type 2 Diabetes Influences Glucose and Lipid Metabolism in Skeletal Muscle
Massart, Sjögren, Lundell et al
Diabetes (2017)
AI Summary: OriGene's pri-miR-29a and pri-miR-29c plasmids were used to overexpress miR-29 in mouse skeletal muscle, enabling the study to demonstrate how increased miR-29 impairs glucose uptake and lipid metabolism relevant to type 2 diabetes.
Snippet: After one week of acclimatization, tibialis anterior muscle of 12 week old C57BL/6J mice was transfected with either a control plasmid or plasmid encoding for pri-miR-29a or pri-miR-29c (Origene, Rockville, MD) by electroporation as described (13). ... All 3 versions Cite SaveSaving
Showing 1-2 of 2 papers.
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