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MIR196a Rat MicroRNA Expression Plasmid (MI0000940)

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SKU
SC403197
Expression Plasmid of Rat microRNA MIR196a
  $424.00
3 Weeks*

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Specifications
Specifications
Product Data
Species Rat
Vector pCMV-MIR
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequences
Precursor: UGUUUGCUCAGCUGAUCUGUGGCUUAGGUAGUUUCAUGUUGUUGGGAUUGAGUUUUGAACUCGGCAACAAGAAACUGCCUGAGUUACAUCAGUCGGUUUUCGUCGAGGGC:
Mature Sequence: UAGGUAGUUUCAUGUUGUUGGG:
Full Insert
>MI0000940 AACGATCCAGCTCCCCCCAGGGCCAGGGCCAGATGCAGGGGGATAGCGCTATTGCAGTACTGGGGGTGGG
GCAGGGATTTGGCGGCTAGAGGTTCCATTTCACCAGATTTTCTCAATGCAACTTGCTTATACCAGAAACT
AACAGAGGCTATCAGATCCTCAATCCAACTAGCCACCCAAAGTCCACTCTATAGTCCTTAGGGAGGTTGT
AAGGGCGGAAAGGGGATCGGGCTAAATTTCTTCCTTTCCCAAGCCCCTTCCCTTCTCCTTTGGAGAGATG
CAAAGCCTAAGCTCCGGCCCTGTTTGCTCAGCTGATCTGTGGCTTAGGTAGTTTCATGTTGTTGGGATTG
AGTTTTGAACTCGGCAACAAGAAACTGCCTGAGTTACATCAGTCGGTTTTCGTCGAGGGCCCCAATCCAC
CTCCCCTAACCCTTCCCTCCCCTGTGGGGCTGCCCCACCAGCAATCCCAGATAGGGCAAAATGGGTGCAG
ACTGAGGAGGTCAGGCTGTGAGTGGGGCCATGGGACAAATTCTGGACAGCCCTGCTTTACAACCATCTCC
CCCTCAGCCTGTCCCAATCTCTGGAACAGAGGGAAAGCCCCCCCCCCAGCTATTGTCTCCTGAGCCCAGC
TGATAGAGACAGACAAAGAAGGTCCAACCAATTCCAAGGAGGTGCAGGAGTTTGAGTCTGGCCTGGCGGG
CCAGCTTAGT
Components 1 x 10 ug vial miRNA plasmid lyophilized in a 2-D bar-coded Matrix tube
Also Contains Both mature and minor (star) miRNA can be over expressed from the construct.
OTI Disclaimer All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
Shipping Ambient
Reference Data
RefSeq MI0000940
Synonyms rno-mir-196a
Locus ID 100314048
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Documents
Resources

file-alt Citations

Abstracts of Scientific Paper (Nepal, Philippines, Singapore, Taiwan, Thailand)
OC
SCIENTIFIC PAPER COMPETITION (2014)
AI Summary: OriGene's pCMV-MIR vector, containing a GFP reporter gene, was used to construct and express miR-196a2 plasmids for transfection experiments in the study.
Snippet: … Vector pCMV-MIR ( Origene , Rockville, MD) including a green fluorescein protein (GFP) reporter gene was used to construct the miR-196a2-C plasmid. A C to T mutation was introduced to the miR-196a2 plasmid at rs11614913, generating miR-196a2-T, by using the QuikChange II site-directed mu tagenesis kit (Agilent Technologies, Inc.;Santa Clara, CA). …
Showing 1-1 of 1 papers.
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